ArticleMolecular and cellular biology2010
Comparative analysis of chromatin binding by Sex Comb on Midleg (SCM) and other polycomb group repressors at a Drosophila Hox gene.
Article in Molecular and cellular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 45 citations in OpenAlex.
- Discordant interactions between YAP1 and polycomb group protein SCML2 determine cell fate.iScience · 2023Article
- Polycomb Recruiters Inside and Outside of the Repressed Domains.International journal of molecular sciences · 2023Review
- Sex Comb on Midleg Like-2 Accelerates Hepatocellular Carcinoma Cell Proliferation and Metastasis by Activating Wnt/β-Catenin/EMT Signaling.Yonsei medical journal · 2021Article
- SUMOylation in development and neurodegeneration.Development (Cambridge, England) · 2020Review
- Article
- Epigenetic regulation of HIV-1 latency: focus on polycomb group (PcG) proteins.Clinical epigenetics · 2018Review
- Sex comb on midleg like-2 is a novel specific marker for the diagnosis of gastroenteropancreatic neuroendocrine tumors.Experimental and therapeutic medicine · 2017Article
- Molecular basis of PRC1 targeting to Polycomb response elements by PhoRC.Genes & development · 2016Article
- Adaptive selection and coevolution at the proteins of the Polycomb repressive complexes in Drosophila.Heredity · 2016Article
- Trithorax regulates systemic signaling during Drosophila imaginal disc regeneration.Development (Cambridge, England) · 2015Article
- The Arabidopsis GAGA-Binding Factor BASIC PENTACYSTEINE6 Recruits the POLYCOMB-REPRESSIVE COMPLEX1 Component LIKE HETEROCHROMATIN PROTEIN1 to GAGA DNA Motifs.Plant physiology · 2015Article
- Sex comb on midleg (Scm) is a functional link between PcG-repressive complexes in Drosophila.Genes & development · 2015Article
- USP7 cooperates with SCML2 to regulate the activity of PRC1.Molecular and cellular biology · 2015Article
- Polycomb group genes are required to maintain a binary fate choice in the Drosophila eye.Neural development · 2015Article
- Polycomb protein SCML2 associates with USP7 and counteracts histone H2A ubiquitination in the XY chromatin during male meiosis.PLoS genetics · 2015Article
- Interactions with RNA direct the Polycomb group protein SCML2 to chromatin where it represses target genes.eLife · 2014Article
- Combinatorial interactions are required for the efficient recruitment of pho repressive complex (PhoRC) to polycomb response elements.PLoS genetics · 2014Article
- Solution NMR structure of the DNA-binding domain from Scml2 (sex comb on midleg-like 2).The Journal of biological chemistry · 2014Article
- Are we there yet? Initial targeting of the Male-Specific Lethal and Polycomb group chromatin complexes in Drosophila.Open biology · 2014Review
- Polycomb protein SCML2 regulates the cell cycle by binding and modulating CDK/CYCLIN/p21 complexes.PLoS biology · 2013Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Sex Comb on Midleg (SCM) is a transcriptional repressor in the Polycomb group (PcG), but its molecular role in PcG silencing is not known. Although SCM can interact with Polycomb repressive complex 1 (PRC1) in vitro, biochemical studies have indicated that SCM is not a core constituent of PRC1 or PRC2. Nevertheless, SCM is just as critical for Drosophila Hox gene silencing as canonical subunits of these well-characterized PcG complexes. To address functional relationships between SCM and other PcG components, we have performed chromatin immunoprecipitation studies using cultured Drosophila Schneider line 2 (S2) cells and larval imaginal discs. We find that SCM associates with a Polycomb response element (PRE) upstream of the Ubx gene which also binds PRC1, PRC2, and the DNA-binding PcG protein Pleiohomeotic (PHO). However, SCM is retained at this Ubx PRE despite genetic disruption or knockdown of PHO, PRC1, or PRC2, suggesting that SCM chromatin targeting does not require prior association of these other PcG components. Chromatin immunoprecipitations (IPs) to test the consequences of SCM genetic disruption or knockdown revealed that PHO association is unaffected, but reduced levels of PRE-bound PRC2 and PRC1 were observed. We discuss these results in light of current models for recruitment of PcG complexes to chromatin targets.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.