ArticleMolecular and cellular biology2010
Genetic analysis of hierarchical regulation for Gata1 and NF-E2 p45 gene expression in megakaryopoiesis.
Article in Molecular and cellular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- Autophagy and its consequences for platelet biology.Thrombosis research · 2023Review
- The Application of Ethnomedicine in Modulating Megakaryocyte Differentiation and Platelet Counts.International journal of molecular sciences · 2023Review
- TMEA, a Polyphenol inFrontiers in cell and developmental biology · 2021Article
- Membrane budding is a major mechanism of in vivo platelet biogenesis.The Journal of experimental medicine · 2020Article
- Immunomodulatory drugs downregulate IKZF1 leading to expansion of hematopoietic progenitors with concomitant block of megakaryocytic maturation.Haematologica · 2018Article
- The KEAP1-NRF2 System: a Thiol-Based Sensor-Effector Apparatus for Maintaining Redox Homeostasis.Physiological reviews · 2018Review
- A novel mode of stimulating platelet formation activity in megakaryocytes with peanut skin extract.Journal of natural medicines · 2018Article
- Estrogen promotes megakaryocyte polyploidization via estrogen receptor beta-mediated transcription of GATA1.Leukemia · 2017Article
- Review
- Role of autophagy in megakaryocyte differentiation and platelet formation.International journal of physiology, pathophysiology and pharmacology · 2016Review
- Manipulating megakaryocytes to manufacture platelets ex vivo.Journal of thrombosis and haemostasis : JTH · 2015Review
- Regulation and function of the NFE2 transcription factor in hematopoietic and non-hematopoietic cells.Cellular and molecular life sciences : CMLS · 2015Review
- Selective deletion of Jak2 in adult mouse hematopoietic cells leads to lethal anemia and thrombocytopenia.Haematologica · 2014Article
- Platelets and cancer: a casual or causal relationship: revisited.Cancer metastasis reviews · 2014Review
- NF-E2 p45 is important for establishing normal function of platelets.Molecular and cellular biology · 2013Article
- Transcription factors in late megakaryopoiesis and related platelet disorders.Journal of thrombosis and haemostasis : JTH · 2013Review
- N- and C-terminal transactivation domains of GATA1 protein coordinate hematopoietic program.The Journal of biological chemistry · 2012Article
- Isolation and characterization of renal erythropoietin-producing cells from genetically produced anemia mice.PloS one · 2011Article
- Hierarchical differentiation of myeloid progenitors is encoded in the transcription factor network.PloS one · 2011Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
GATA1 and NF-E2 p45 are two important regulators of megakaryopoiesis. Whereas GATA1 is known to regulate the p45 gene, details of the GATA1 contribution to the spatiotemporal expression of the p45 gene remain to be elucidated. To clarify the relationship between GATA1 and p45, we performed genetic complementation rescue analysis of p45 function in megakaryocytes utilizing the hematopoietic regulatory domain of the Gata1 gene (G1HRD). We established transgenic mouse lines expressing p45 under G1HRD regulation and crossed the mice with p45-null mice. Compound mutant mice displayed normal platelet counts and no sign of hemorrhage, indicating that G1HRD has the ability to express p45 in a spatiotemporally correct manner. However, deletion of 38 amino acids from the N-terminal region of p45 abrogated the p45 rescue function, suggesting the presence of an essential transactivation activity in the region. We then crossed the G1HRD-p45 transgenic mice with megakaryocyte-specific Gata1 gene knockdown (Gata1(Delta)(neo)(Delta)(HS)) mice. The G1HRD-p45 transgene was insufficient for complete rescue of the Gata1(Delta)(neo)(Delta)(HS) megakaryocytes, suggesting that GATA1 or other factors regulated by GATA1 are required to cooperate with p45 for normal megakaryopoiesis. This study thus provides a unique in vivo validation of the hierarchical relationship between GATA1 and p45 in megakaryocytes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.