ArticleInfection and immunity2010
Genome-wide transposon mutagenesis reveals a role for pO157 genes in biofilm development in Escherichia coli O157:H7 EDL933.
Article in Infection and immunity, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
42 citing papers in PubMed, 77 citations in OpenAlex.
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- The role of cydB gene in the biofilm formation by Campylobacter jejuni.Scientific reports · 2024Article
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- Identification and characterization of Acinetobacter nosocomialis BfmRS, two-component regulatory system, essential for biofilm development.Genes & genomics · 2024Article
- Prevalence and Characteristics of Plasmid-Encoded Serine Protease EspP in Clinical Shiga Toxin-ProducingMicroorganisms · 2024Article
- Contrasting genes conferring short- and long-term biofilm adaptation inMicrobial genomics · 2023Article
- Identification of novel biofilm genes in avian pathogenic Escherichia coli by Tn5 transposon mutant library.World journal of microbiology & biotechnology · 2022Review
- IgG BindsFrontiers in immunology · 2022Article
- Outer Membrane Protein F Is Involved in Biofilm Formation, Virulence and Antibiotic Resistance inMicroorganisms · 2021Article
- Massively parallel transposon mutagenesis identifies temporally essential genes for biofilm formation inMicrobial genomics · 2021Article
- Genome-wide genetic marker analysis and genotyping of Escherichia fergusonii strain OTSVEF-60.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021Article
- Biofilm Formation byApplied and environmental microbiology · 2021Article
- Genetic and Phenotypic Factors Associated with Persistent Shedding of Shiga Toxin-Producing Escherichia coli by Beef Cattle.Applied and environmental microbiology · 2020Article
- Regulation ofJournal of bacteriology · 2019Article
- Factors Involved in the Persistence of a Shiga Toxin-ProducingFrontiers in microbiology · 2018Article
- A Method for Quantification of Epithelium Colonization Capacity by Pathogenic Bacteria.Frontiers in cellular and infection microbiology · 2018Article
- Virulence and transcriptome profile of multidrug-resistant Escherichia coli from chicken.Scientific reports · 2017Article
- Large-Scale Transposition Mutagenesis of Streptomyces coelicolor Identifies Hundreds of Genes Influencing Antibiotic Biosynthesis.Applied and environmental microbiology · 2017Article
- Curli Temper Adherence of Escherichia coli O157:H7 to Squamous Epithelial Cells from the Bovine Recto-Anal Junction in a Strain-Dependent Manner.Applied and environmental microbiology · 2017Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enterohemorrhagic Escherichia coli O157:H7, a world-wide human food-borne pathogen, causes mild to severe diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome. The ability of this pathogen to persist in the environment contributes to its dissemination to a wide range of foods and food processing surfaces. Biofilms are thought to be involved in persistence, but the process of biofilm formation is complex and poorly understood in E. coli O157:H7. To better understand the genetics of this process, a mini-Tn5 transposon insertion library was constructed in strain EDL933 and screened for biofilm-negative mutants using a microtiter plate assay. Ninety-five of 11,000 independent insertions (0.86%) were biofilm negative, and transposon insertions were located in 51 distinct genes/intergenic regions that must be involved either directly or indirectly in biofilm formation. All of the 51 biofilm-negative mutants showed reduced biofilm formation on both hydrophilic and hydrophobic surfaces. Thirty-six genes were unique to this study, including genes on the virulence plasmid pO157. The type V secreted autotransporter serine protease EspP and the enterohemolysin translocator EhxD were found to be directly involved in biofilm formation. In addition, EhxD and EspP were also important for adherence to T84 intestinal epithelial cells, suggesting a role for these genes in tissue interactions in vivo.
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Registered trials
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