ArticleNucleic acids research2010
Mechanism of sequence-specific template binding by the DNA primase of bacteriophage T7.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 28 citations in OpenAlex.
- Mechanism of T7 Primase Selecting Active Priming Sites Among Genome.Biomolecules · 2026Article
- Definition of the binding specificity of the T7 bacteriophage primase by analysis of a protein binding microarray using a thermodynamic model.Nucleic acids research · 2024Article
- Article
- Inferring primase-DNA specific recognition using a data driven approach.Nucleic acids research · 2021Article
- Bi- and Multi-directional Gene Transfer in the Natural Populations of Polyvalent Bacteriophages, and Their Host Species Spectrum Representing Foodborne Versus Other Human and/or Animal Pathogens.Food and environmental virology · 2021Article
- DnaG Primase-A Target for the Development of Novel Antibacterial Agents.Antibiotics (Basel, Switzerland) · 2018Review
- Article
- Plant organellar DNA primase-helicase synthesizes RNA primers for organellar DNA polymerases using a unique recognition sequence.Nucleic acids research · 2017Article
- Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins.Journal of visualized experiments : JoVE · 2017Article
- Primer release is the rate-limiting event in lagging-strand synthesis mediated by the T7 replisome.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Insight into the Human DNA Primase Interaction with Template-Primer.The Journal of biological chemistry · 2016Article
- The N-terminal domain of the Drosophila mitochondrial replicative DNA helicase contains an iron-sulfur cluster and binds DNA.The Journal of biological chemistry · 2014Article
- Impact of macromolecular crowding on DNA replication.Nature communications · 2013Article
- Zinc-binding domain of the bacteriophage T7 DNA primase modulates binding to the DNA template.The Journal of biological chemistry · 2012Article
- Heterohexamer of 56- and 63-kDa Gene 4 Helicase-Primase of Bacteriophage T7 in DNA Replication.The Journal of biological chemistry · 2012Article
- The roles of tryptophans in primer synthesis by the DNA primase of bacteriophage T7.The Journal of biological chemistry · 2012Article
- Gene 5.5 protein of bacteriophage T7 in complex with Escherichia coli nucleoid protein H-NS and transfer RNA masks transfer RNA priming in T7 DNA replication.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Choreography of bacteriophage T7 DNA replication.Current opinion in chemical biology · 2011Review
- Direct role for the RNA polymerase domain of T7 primase in primer delivery.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Genome Analysis of an Enterococcal Prophage, Entfac.MY.Avicenna journal of medical biotechnologyArticle
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
DNA primases catalyze the synthesis of the oligoribonucleotides required for the initiation of lagging strand DNA synthesis. Biochemical studies have elucidated the mechanism for the sequence-specific synthesis of primers. However, the physical interactions of the primase with the DNA template to explain the basis of specificity have not been demonstrated. Using a combination of surface plasmon resonance and biochemical assays, we show that T7 DNA primase has only a slightly higher affinity for DNA containing the primase recognition sequence (5'-TGGTC-3') than for DNA lacking the recognition site. However, this binding is drastically enhanced by the presence of the cognate Nucleoside triphosphates (NTPs), Adenosine triphosphate (ATP) and Cytosine triphosphate (CTP) that are incorporated into the primer, pppACCA. Formation of the dimer, pppAC, the initial step of sequence-specific primer synthesis, is not sufficient for the stable binding. Preformed primers exhibit significantly less selective binding than that observed with ATP and CTP. Alterations in subdomains of the primase result in loss of selective DNA binding. We present a model in which conformational changes induced during primer synthesis facilitate contact between the zinc-binding domain and the polymerase domain.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.