ArticleMolecular pharmaceutics2010
Polycation-induced cell membrane permeability does not enhance cellular uptake or expression efficiency of delivered DNA.
Article in Molecular pharmaceutics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 39 citations in OpenAlex.
- Review
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- Achieving efficient clonal beta cells transfection using nanostraw/nanopore-assisted electroporation.RSC advances · 2024Article
- Role of Hydrophobic Modification in Spermine-Based Poly(β-amino ester)s for siRNA Delivery and Their Spray-Dried Powders for Inhalation and Improved Storage.Biomacromolecules · 2024Article
- Non-viral delivery of CRISPR-Cas9 complexes for targeted gene editing via a polymer delivery system.Gene therapy · 2022Article
- Targeting the Inside of Cells with Biologicals: Chemicals as a Delivery Strategy.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2021Review
- Inhibition of aquaporins as a potential adjunct to breast cancer cryotherapy.Oncology letters · 2021Article
- Ionic liquids: prospects for nucleic acid handling and delivery.Nucleic acids research · 2021Review
- Arginine-glycine-aspartate (RGD)-targeted positron-labeled dendritic polylysine nanoprobe for tumor PET imaging.RSC advances · 2020Article
- Article
- Scavenging Circulating Mitochondrial DNA as a Potential Therapeutic Option for Multiple Organ Dysfunction in Trauma Hemorrhage.Frontiers in immunology · 2018Observational
- An easy gene assembling strategy for light-promoted transfection by combining host-guest interaction of cucurbit[7]uril and gold nanoparticles.Scientific reports · 2017Article
- Role of Cell Membrane-Vector Interactions in Successful Gene Delivery.Accounts of chemical research · 2016Article
- Cationic Polymer Intercalation into the Lipid Membrane Enables Intact Polyplex DNA Escape from Endosomes for Gene Delivery.Molecular pharmaceutics · 2016Article
- A Low Protein Binding Cationic Poly(2-oxazoline) as Non-Viral Vector.Macromolecular bioscience · 2015Article
- Quantitative Measurement of Cationic Polymer Vector and Polymer-pDNA Polyplex Intercalation into the Cell Plasma Membrane.ACS nano · 2015Article
- Polyplex exposure inhibits cell cycle, increases inflammatory response, and can cause protein expression without cell division.Molecular pharmaceutics · 2013Article
- Exploring the mechanism of plasmid DNA nuclear internalization with polymer-based vehicles.Molecular pharmaceutics · 2012Article
- Best practices for purification and characterization of PAMAM dendrimer.Macromolecules · 2012Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Polycationic materials commonly used to delivery DNA to cells are known to induce cell membrane porosity in a charge-density dependent manner. It has been suggested that these pores may provide a mode of entry of the polymer-DNA complexes (polyplexes) into cells. To examine the correlation between membrane permeability and biological activity, we used two-color flow cytometry on two mammalian cell lines to simultaneously measure gene expression of a plasmid DNA delivered with four common nonviral vectors and cellular uptake of normally excluded fluorescent dye molecules of two different sizes, 668 Da and 2 MDa. We also followed gene expression in cells sorted based on the retention of endogenous fluorescein. We have found that cell membrane porosity caused by polycationic vectors does not enhance internalization or gene expression. Based on this single-cell study, membrane permeability is found to be an unwanted side effect that limits transfection efficiency, possibly through leakage of the delivered nucleic acid through the pores prior to transcription and translation and/or activation of cell defense mechanisms that restrict transgene expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.