ArticleMolecular biology reports2011
Molecular analysis shows differential expression of R-spondin1 in zebrafish (Danio rerio) gonads.
Article in Molecular biology reports, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 45 citations in OpenAlex.
- Article
- Article
- R-spondin family biology and emerging linkages to cancer.Annals of medicine · 2023Review
- Potential antagonistic relationship ofFrontiers in molecular biosciences · 2022Article
- Becoming female: Ovarian differentiation from an evolutionary perspective.Frontiers in cell and developmental biology · 2022Review
- R-Spondin1 enhances wnt signaling and decreases weight loss in short bowel syndrome zebrafish.Biochemistry and biophysics reports · 2021Article
- Article
- Dimorphic expression of sex-related genes in different gonadal development stages of sterlet, Acipenser ruthenus, a primitive fish species.Fish physiology and biochemistry · 2017Article
- R-spondin 1 is required for specification of hematopoietic stem cells through Wnt16 and Vegfa signaling pathways.Development (Cambridge, England) · 2017Article
- Transcriptome analysis of the gonads of olive flounder (Paralichthys olivaceus).Fish physiology and biochemistry · 2016Article
- Rspo1-activated signalling molecules are sufficient to induce ovarian differentiation in XY medaka (Oryzias latipes).Scientific reports · 2016Article
- Plasticity of gene-regulatory networks controlling sex determination: of masters, slaves, usual suspects, newcomers, and usurpators.EMBO reports · 2015Review
- Small-scale transcriptomics reveals differences among gonadal stages in Asian seabass (Lates calcarifer).Reproductive biology and endocrinology : RB&E · 2014Article
- Expression and sequence evolution of aromatase cyp19a1 and other sexual development genes in East African cichlid fishes.Molecular biology and evolution · 2013Article
- Divergent expression regulation of gonad development genes in medaka shows incomplete conservation of the downstream regulatory network of vertebrate sex determination.Molecular biology and evolution · 2013Article
- Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
R-spondin1 (RSPO1) is a potential female-determining gene in human (Homo sapiens) and mouse (Mus musculus). Its differential expression in these mammals is correlated with signaling for sex determination. As a way of studying sex determination in fish we cloned and analyzed a RSPO1 gene in zebrafish (Danio rerio). Using real-time PCR, we observed that RSPO1 is expressed more strongly in ovaries than in testes, suggesting that RSPO1 may have a role in gonad differentiation. High RSPO1 expression was detected in some non-gonadal organs like muscle and kidneys. In situ hybridization results demonstrate that RSPO1 is expressed in premature germ cells, in oogonia and primary oocytes in ovaries and in spermatogonia and spermatocytes in testes. It is also expressed in gonad somatic cells during gonadal development: in granulosa cells and theca cells of early and late cortical-alveolar stage follicles in ovaries, and in Leydig cells in testes. This differential expression may indicate that RSPO1 has a role(s) in zebrafish gonad development and differentiation. By fusing zebrafish RSPO1 with a green fluorescent protein gene, we found that RSPO1 is located in the cytosol and Golgi apparatus but not the nucleus of fish epithelioma papulosum cyprinid (EPC) cells. These preliminary findings suggest some aspects of RSPO1 like differential expression linked to sex determination may be conserved in fish while other aspects like subcellular localization differ from the mammalian RSPO1.
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