ArticleRNA (New York, N.Y.)2010
Regulation of pre-mRNA splicing in Xenopus oocytes by targeted 2'-O-methylation.
Article in RNA (New York, N.Y.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 39 citations in OpenAlex.
- Nuclear 2'-Science advances · 2025Article
- Evolutionary Origins and Functional Diversification of 2'-O-Methyltransferases: Insights from Phylogenetic and Structural Analysis.International journal of molecular sciences · 2025Article
- Suppressor tRNA in gene therapy.Science China. Life sciences · 2024Review
- 2'-RNA (New York, N.Y.) · 2024Review
- An integrative platform for detection of RNA 2'-O-methylation reveals its broad distribution on mRNA.Cell reports methods · 2024Article
- RNA methylation in inflammatory bowel disease.Cancer science · 2024Review
- Regulation and functions of non-mNature reviews. Molecular cell biology · 2023Review
- Novel sights on therapeutic, prognostic, and diagnostics aspects of non-coding RNAs in glioblastoma multiforme.Metabolic brain disease · 2023Review
- RNA Epigenetics in Chronic Lung Diseases.Genes · 2022Review
- Review
- From Antisense RNA to RNA Modification: Therapeutic Potential of RNA-Based Technologies.Biomedicines · 2021Review
- Molecular mechanism of RNase R substrate sensitivity for RNA ribose methylation.Nucleic acids research · 2021Article
- A mark of disease: how mRNA modifications shape genetic and acquired pathologies.RNA (New York, N.Y.) · 2021Review
- Roles of RNA Methylation on Tumor Immunity and Clinical Implications.Frontiers in immunology · 2021Review
- 2'-O-Methylation can increase the abundance and lifetime of alternative RNA conformational states.Nucleic acids research · 2020Article
- Pre-mRNA modifications and their role in nuclear processing.Quantitative biology (Beijing, China) · 2018Article
- Biology and clinical relevance of noncoding sno/scaRNAs.Trends in cardiovascular medicine · 2018Review
- Illumina-based RiboMethSeq approach for mapping of 2'-O-Me residues in RNA.Nucleic acids research · 2016Article
- Dual function of C/D box small nucleolar RNAs in rRNA modification and alternative pre-mRNA splicing.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Remarkable acceleration of a DNA/RNA inter-strand functionality transfer reaction to modify a cytosine residue: the proximity effect via complexation with a metal cation.Nucleic acids research · 2014Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
The 2'-OH group of the branch point adenosine is a key moiety to initiate pre-mRNA splicing. We use RNA-guided RNA modification to target the pre-mRNA branch point adenosine for 2'-O-methylation, with the aim of blocking pre-mRNA splicing in vertebrate cells. We show that, under certain conditions, injection of a branch point-specific artificial box C/D RNA into Xenopus oocytes effectively 2'-O-methylates adenovirus pre-mRNA at the target nucleotide. However, 2'-O-methylation at the authentic branch point activates a host of cryptic branch points, thus allowing splicing to continue. These cryptic sites are mapped, and mutated. Upon injection, pre-mRNA free of cryptic branch points fails to splice when the branch point-specific box C/D RNA is present. However, 2'-O-methylation at the branch point does not prevent pre-mRNA from being assembled into pre-catalytic spliceosome-like complexes prior to the first chemical step of splicing. Our results demonstrate that RNA-guided pre-mRNA modification can occur in the nucleoplasm of vertebrate cells, thus offering a powerful tool for molecular biology research.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.