ArticleRNA (New York, N.Y.)2010
Role of the C-terminal tail of SmpB in the early stage of trans-translation.
Article in RNA (New York, N.Y.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 32 citations in OpenAlex.
- Review
- Ribosome Rescue Pathways in Bacteria.Frontiers in microbiology · 2021Review
- Ribosome-based quality control of mRNA and nascent peptides.Wiley interdisciplinary reviews. RNA · 2017Review
- Targeting Inhibition of SmpB by Peptide Aptamer Attenuates the Virulence to Protect Zebrafish againstFrontiers in microbiology · 2017Article
- Genetic Selection of Peptide Aptamers That Interact and Inhibit Both Small Protein B and Alternative Ribosome-Rescue Factor A of Aeromonas veronii C4.Frontiers in microbiology · 2016Article
- Mechanisms of ribosome rescue in bacteria.Nature reviews. Microbiology · 2015Review
- ArfA recognizes the lack of mRNA in the mRNA channel after RF2 binding for ribosome rescue.Nucleic acids research · 2014Article
- Rejection of tmRNA·SmpB after GTP hydrolysis by EF-Tu on ribosomes stalled on intact mRNA.RNA (New York, N.Y.) · 2014Article
- Resolving nonstop translation complexes is a matter of life or death.Journal of bacteriology · 2014Review
- Identification of residues required for stalled-ribosome rescue in the codon-independent release factor YaeJ.Nucleic acids research · 2014Article
- An unusual mechanism for EF-Tu activation during tmRNA-mediated ribosome rescue.RNA (New York, N.Y.) · 2014Article
- Trans-translation exposed: understanding the structures and functions of tmRNA-SmpB.Frontiers in microbiology · 2014Review
- Mechanism of trans-translation revealed by in vitro studies.Frontiers in microbiology · 2014Review
- tmRNA-mediated trans-translation as the major ribosome rescue system in a bacterial cell.Frontiers in genetics · 2014Review
- The SmpB C-terminal tail helps tmRNA to recognize and enter stalled ribosomes.Frontiers in microbiology · 2014Review
- Structural basis for the rescue of stalled ribosomes: structure of YaeJ bound to the ribosome.Science (New York, N.Y.) · 2012Article
- Decoding in the absence of a codon by tmRNA and SmpB in the ribosome.Science (New York, N.Y.) · 2012Article
- The tmRNA ribosome-rescue system.Advances in protein chemistry and structural biology · 2012Review
- The role of SmpB and the ribosomal decoding center in licensing tmRNA entry into stalled ribosomes.RNA (New York, N.Y.) · 2011Article
- YaeJ is a novel ribosome-associated protein in Escherichia coli that can hydrolyze peptidyl-tRNA on stalled ribosomes.Nucleic acids research · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Trans-translation relieves a stalled translation on the bacterial ribosome by transfer-messenger RNA (tmRNA) with the help of SmpB, an essential cofactor of tmRNA. Here, we examined the role of the unstructured C-terminal tail of SmpB using an in vitro trans-translation system. It was found that truncation of the C-terminal tail or substitution of tryptophan residue at 147 in the middle of the C-terminal tail affected the activity in the early stage of trans-translation. Our investigations also revealed that the C-terminal tail is not required for the events until GTP is hydrolyzed by EF-Tu in complex with tmRNA-SmpB. A synthetic peptide corresponding to the C-terminal tail of SmpB inhibited peptidyl-transfer of alanyl-tmRNA and A-site binding of SmpB, but not GTP hydrolysis. These results suggest that the C-terminal tail has a role in the step of accommodation of alanyl-tmRNA-SmpB into the A-site. Directed hydroxyl radical probing indicated that tryptophan residue at 147 is located just downstream of the decoding center in the mRNA path when SmpB is in the A-site.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.