ArticleEukaryotic cell2010
Analysis of the high-affinity iron uptake system at the Chlamydomonas reinhardtii plasma membrane.
Article in Eukaryotic cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 34 citations in OpenAlex.
- Siderophore production by the lichen fungus Xanthoria parietina supports its algal symbiont.Nature communications · 2026Article
- APX2 Is an Ascorbate Peroxidase-Related Protein that Regulates the Levels of Plastocyanin in Chlamydomonas.Plant & cell physiology · 2024Article
- Short-term response to iron resupply in an iron-limited open ocean diatom reveals rapid decay of iron-responsive transcripts.PloS one · 2023Article
- Optimizing Fe Nutrition for Algal Growth.Methods in molecular biology (Clifton, N.J.) · 2023Article
- A quick journey into the diversity of iron uptake strategies in photosynthetic organisms.Plant signaling & behavior · 2021Review
- Regulation of Iron Homeostasis and Use in Chloroplasts.International journal of molecular sciences · 2020Review
- Regulating cellular trace metal economy in algae.Current opinion in plant biology · 2017Review
- CrMAPK3 regulates the expression of iron-deficiency-responsive genes in Chlamydomonas reinhardtii.BMC biochemistry · 2017Article
- Ni induces the CRR1-dependent regulon revealing overlap and distinction between hypoxia and Cu deficiency responses in Chlamydomonas reinhardtii.Metallomics : integrated biometal science · 2016Article
- Iron economy in Chlamydomonas reinhardtii.Frontiers in plant science · 2013Review
- Multi-copper oxidases and human iron metabolism.Nutrients · 2013Review
- Iron metabolism in aerobes: managing ferric iron hydrolysis and ferrous iron autoxidation.Coordination chemistry reviews · 2013Article
- The ins and outs of algal metal transport.Biochimica et biophysica acta · 2012Review
- Multicopper oxidase-1 is a ferroxidase essential for iron homeostasis in Drosophila melanogaster.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Disassembling iron availability to phytoplankton.Frontiers in microbiology · 2012Article
- Functional characterization of the ferroxidase, permease high-affinity iron transport complex from Candida albicans.Molecular microbiology · 2011Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Multicopper ferroxidases play a vital role in iron metabolism in bacteria, fungi, algae, and mammals. Saccharomyces cerevisiae utilizes a channeling mechanism to couple the ferroxidase activity of Fet3p to Fe(3+) transport into the cell by Ftr1p. In contrast, the mechanisms by which mammals couple the ferroxidase reaction to iron trafficking is unclear. The human ferroxidases ceruloplasmin and hephaestin are twice the size of Fet3p and interact with proteins that are not expressed in fungi. Chlamydomonas FOX1 is a homolog of the human ferroxidases but likely supports iron uptake in a manner similar to that of yeast, since Chlamydomonas reinhardtii expresses a ferric iron permease homolog, FTR1. The results presented support this hypothesis. We show that FOX1 is trafficked to the plasma membrane and is oriented with its multicopper oxidase/ferroxidase domain in the exocytoplasmic space. Our analysis of FTR1 indicates its topology is similar to that of S. cerevisiae Ftr1p, with a potential exocytoplasmic iron channeling motif and two potential iron permeation motifs in membrane-spanning regions. We demonstrate that high-affinity iron uptake is dependent on FOX1 and the copper status of the cell. Kinetic inhibition of high-affinity iron uptake by a ferric iron chelator does not reflect the strength of the chelator, supporting a ferric iron channeling mechanism for high-affinity iron uptake in Chlamydomonas. Last, recombinant FOX1 (rFOX1) has been isolated in a partially holo form that exhibits the UV-visible absorbance spectrum of a multicopper oxidase and the catalytic activity of a ferroxidase.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.