ArticleEukaryotic cell2010
Characterization of the Roco protein family in Dictyostelium discoideum.
Article in Eukaryotic cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 39 citations in OpenAlex.
- Non-Mammalian Models for Mitochondria Research in CNS Disorders.Biomolecules · 2026Review
- LRRK2 kinase activity is necessary for development and regeneration in Nematostella vectensis.Research square · 2023Article
- Review
- Interactome and evolutionary conservation of Dictyostelid small GTPases and their direct regulators.Small GTPases · 2022Article
- A Conserved Role for LRRK2 and Roco Proteins in the Regulation of Mitochondrial Activity.Frontiers in cell and developmental biology · 2021Article
- Akt and SGK protein kinases are required for efficient feeding by macropinocytosis.Journal of cell science · 2019Article
- Roco Proteins: GTPases with a Baroque Structure and Mechanism.International journal of molecular sciences · 2019Review
- The dual enzyme LRRK2 hydrolyzes GTP in both its GTPase and kinase domains in vitro.Biochimica et biophysica acta. Proteins and proteomics · 2017Article
- Structural model of the dimeric Parkinson's protein LRRK2 reveals a compact architecture involving distant interdomain contacts.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Activation Mechanism of LRRK2 and Its Cellular Functions in Parkinson's Disease.Parkinson's disease · 2016Review
- Ten years and counting: moving leucine-rich repeat kinase 2 inhibitors to the clinic.Movement disorders : official journal of the Movement Disorder Society · 2015Review
- Genetic, structural, and molecular insights into the function of ras of complex proteins domains.Chemistry & biology · 2014Review
- SILAC-based proteomic quantification of chemoattractant-induced cytoskeleton dynamics on a second to minute timescale.Nature communications · 2014Article
- Structural biology of the LRRK2 GTPase and kinase domains: implications for regulation.Frontiers in molecular neuroscience · 2014Review
- Roco kinase structures give insights into the mechanism of Parkinson disease-related leucine-rich-repeat kinase 2 mutations.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Multiple regulatory mechanisms for the Dictyostelium Roco protein GbpC.The Journal of biological chemistry · 2012Article
- The LRRK2-related Roco kinase Roco2 is regulated by Rab1A and controls the actin cytoskeleton.Molecular biology of the cell · 2011Article
- Signaling mechanisms for chemotaxis.Development, growth & differentiation · 2011Review
- The ROCO kinase QkgA is necessary for proliferation inhibition by autocrine signals in Dictyostelium discoideum.Eukaryotic cell · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Roco family consists of multidomain Ras-GTPases that include LRRK2, a protein mutated in familial Parkinson's disease. The genome of the cellular slime mold Dictyostelium discoideum encodes 11 Roco proteins. To study the functions of these proteins, we systematically knocked out the roco genes. Previously described functions for GbpC, Pats1, and QkgA (Roco1 to Roco3) were confirmed, while novel developmental defects were identified in roco4- and roco11-null cells. Cells lacking Roco11 form larger fruiting bodies than wild-type cells, while roco4-null cells show strong developmental defects during the transition from mound to fruiting body; prestalk cells produce reduced levels of cellulose, leading to unstable stalks that are unable to properly lift the spore head. Detailed phylogenetic analysis of four slime mold species reveals that QkgA and Roco11 evolved relatively late by duplication of an ancestor roco4 gene (later than approximately 300 million years ago), contrary to the situation with other roco genes, which were already present before the split of the common ancestor of D. discoideum and Polysphondylium pallidum (before approximately 600 million years ago). Together, our data show that the Dictyostelium Roco proteins serve a surprisingly diverse set of functions and highlight Roco4 as a key protein for proper stalk cell formation.
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