ArticleApplied and environmental microbiology2010
The internal transcribed spacer region, a new tool for use in species differentiation and delineation of systematic relationships within the Campylobacter genus.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- NewStudies in mycology · 2024Article
- The Contribution of the Human Oral Microbiome to Oral Disease: A Review.Microorganisms · 2023Review
- High-resolution phylogenetic and population genetic analysis of microbial communities with RoC-ITS.ISME communications · 2022Article
- Exposure to bacterial and fungal bioaerosols in facilities processing biodegradable waste.Frontiers in public health · 2022Article
- Isolation and cultivation of candidate phyla radiationJournal of oral microbiology · 2020Article
- Survival Mechanisms ofFrontiers in microbiology · 2019Article
- Comparative genomics of Campylobacter concisus: Analysis of clinical strains reveals genome diversity and pathogenic potential.Emerging microbes & infections · 2018Article
- Inquiring into the Gaps of Campylobacter Surveillance Methods.Veterinary sciences · 2017Review
- A report on identification of sequence polymorphism in barcode region of six commercially important Cymbopogon species.Molecular biology reports · 2017Article
- 16S-23S rRNA Gene Intergenic Spacer Region Variability Helps Resolve Closely Related Sphingomonads.Frontiers in microbiology · 2016Article
- Global Epidemiology of Campylobacter Infection.Clinical microbiology reviews · 2015Review
- A nucleotide signature for identification of Aglaia stellatopilosa Pannell.Molecular biotechnology · 2014Article
- Differentiation of non-pylori Helicobacter species based on PCR-restriction fragment length polymorphism of the 23S rRNA gene.World journal of microbiology & biotechnology · 2014Article
- Molecular analysis of the 16S-23S rDNA internal spacer region (ISR) and truncated tRNA(Ala) gene segments in Campylobacter lari.World journal of microbiology & biotechnology · 2012Article
- The clinical importance of emerging Campylobacter species.Nature reviews. Gastroenterology & hepatology · 2011Review
- Comparative analyses of Campylobacter concisus strains reveal the genome of the reference strain BAA-1457 is not representative of the species.Gut pathogens · 2011Article
- Sequencing and validation of the genome of a Campylobacter concisus reveals intra-species diversity.PloS one · 2011Article
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Authors and funding
4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The Campylobacter genus consists of a number of important human and animal pathogens. Although the 16S rRNA gene has been used extensively for detection and identification of Campylobacter species, there is currently limited information on the 23S rRNA gene and the internal transcribed spacer (ITS) region that lies between the 16S and 23S rRNA genes. We examined the potential of the 23S rRNA gene and the ITS region to be used in species differentiation and delineation of systematic relationships for 30 taxa within the Campylobacter genus. The ITS region produced the highest mean pairwise percentage difference (35.94%) compared to the 16S (5.34%) and 23S (7.29%) rRNA genes. The discriminatory power for each region was further validated using Simpson's index of diversity (D value). The D values were 0.968, 0.995, and 0.766 for the ITS region and the 23S and 16S rRNA genes, respectively. A closer examination of the ITS region revealed that Campylobacter concisus, Campylobacter showae, and Campylobacter fetus subsp. fetus harbored tRNA configurations not previously reported for other members of the Campylobacter genus. We also observed the presence of strain-dependent intervening sequences in the 23S rRNA genes. Neighbor-joining trees using the ITS region revealed that Campylobacter jejuni and Campylobacter coli strains clustered in subgroups, which was not observed in trees derived from the 16S or 23S rRNA gene. Of the three regions examined, the ITS region is by far the most cost-effective region for the differentiation and delineation of systematic relationships within the Campylobacter genus.
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