ArticleJournal of neuroscience methods2010
Optimal promoter usage for lentiviral vector-mediated transduction of cultured central nervous system cells.
Article in Journal of neuroscience methods, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 62 citations in OpenAlex.
- Article
- Fluorescence-Based HTS Assays for Ion Channel Modulation in Drug Discovery Pipelines.ChemMedChem · 2024Review
- Establishment and Characterization of a Stable Producer Cell Line Generation Platform for the Manufacturing of Clinical-Grade Lentiviral Vectors.Biomedicines · 2024Article
- Combining CRISPR-Cas9 and TCR exchange to generate a safe and efficient cord blood-derived T cell product for pediatric relapsed AML.Journal for immunotherapy of cancer · 2024Article
- Investigating dynamics of lentiviral vector secretion from HEK293T producer cells using a fractionated perfusion system.Biotechnology journal · 2024Article
- Live-cell imaging of microRNA expression with post-transcriptional feedback control.Molecular therapy. Nucleic acids · 2021Article
- The effect of genetically modified platelet-derived growth factor-BB over-expressing mesenchymal stromal cells during core decompression for steroid-associated osteonecrosis of the femoral head in rabbits.Stem cell research & therapy · 2021Article
- Reversal of prolonged obesity-associated cerebrovascular dysfunction by inhibiting microglial Tak1.Nature neuroscience · 2020Article
- Lentiviral vector-mediated transduction of adult neural stem/progenitor cells isolated from the temporal tissues of epileptic patients.Iranian journal of basic medical sciences · 2020Article
- Hypothalamic Sirt1 protects terminal Schwann cells and neuromuscular junctions from age-related morphological changes.Aging cell · 2018Article
- Sensitive and long-term monitoring of intracellular microRNAs using a non-integrating cytoplasmic RNA vector.Scientific reports · 2017Article
- Deletion of the Virion Host Shut-off Gene Enhances Neuronal-Selective Transgene Expression from an HSV Vector Lacking Functional IE Genes.Molecular therapy. Methods & clinical development · 2017Article
- Intermediate filament aggregates cause mitochondrial dysmotility and increase energy demands in giant axonal neuropathy.Human molecular genetics · 2016Article
- Efficient Expression of Igf-1 from Lentiviral Vectors Protects In Vitro but Does Not Mediate Behavioral Recovery of a Parkinsonian Lesion in Rats.Human gene therapy · 2015Article
- Lentiviral vectors as tools to understand central nervous system biology in mammalian model organisms.Frontiers in molecular neuroscience · 2015Review
- Gene delivery strategies to promote spinal cord repair.Biomarker insights · 2015Review
- Pros and Cons of Antigen-Presenting Cell Targeted Tumor Vaccines.Journal of immunology research · 2015Review
- Viral expression cassette elements to enhance transgene target specificity and expression in gene therapy.Discovery medicine · 2015Review
- Sexually dimorphic RB inactivation underlies mesenchymal glioblastoma prevalence in males.The Journal of clinical investigation · 2014Article
- Fast phasic release properties of dopamine studied with a channel biosensor.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
Abstract
Lentiviral vectors transduce both dividing and non-dividing cells and can support sustained expression of transgenes. These properties make them attractive for the transduction of neurons and other neural cell types in vitro and in vivo. Lentiviral vectors can be targeted to specific cell types by using different promoters in the lentiviral shuttle vector. Even with identical constructs, however, levels of expression can vary significantly in different types of neurons and different culture preparations; expression levels in the same neuronal subtypes can be very different in primary cell culture and in vivo. We systematically assessed the ability of different promoters to direct expression of foreign transgenes in primary murine neocortical neurons, cerebellar granule cells and in undifferentiated and differentiated neuroblastoma cells. In primary cortical neurons, constructs using the ubiquitin C promoter directed the highest level of transgene expression; the phosphoglycerate kinase (PGK) promoter also directed robust transgene expression, while the cytomegalovirus (CMV) and MND (a synthetic promoter that contains the U3 region of a modified MoMuLV LTR with myeloproliferative sarcoma virus enhancer) promoters resulted in the expression of the transgenes in only limited number of neurons. In contrast, in cerebellar granule cells and in differentiated SH-SY5Y neuroblastoma cultures, the CMV promoter directed the most robust transgene expression. There was similar variability in transgene expression directed by these promoters in primary cultures of oligodendrocytes and astrocytes. These findings may prove useful in the design of lentiviral vectors for use in cell culture models of the nervous system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.