ArticleBiophysical chemistry2010
Gel mobilities of linking-number topoisomers and their dependence on DNA helical repeat and elasticity.
Article in Biophysical chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 22 citations in OpenAlex.
- Suitability of double-stranded DNA as a molecular standard for the validation of analytical ultracentrifugation instruments.European biophysics journal : EBJ · 2023Article
- DNA supercoiling differences in bacteria result from disparate DNA gyrase activation by polyamines.PLoS genetics · 2020Article
- Loop-closure kinetics reveal a stable, right-handed DNA intermediate in Cre recombination.Nucleic acids research · 2020Article
- Deconvolution of nucleic-acid length distributions: a gel electrophoresis analysis tool and applications.Nucleic acids research · 2019Article
- Dataset on the effects of spermidine on linking number differences between histone H1-free and histone H1-bound circular polynucleosomes.Data in brief · 2018Article
- Quaternary interactions and supercoiling modulate the cooperative DNA binding of AGT.Nucleic acids research · 2017Article
- Structural diversity of supercoiled DNA.Nature communications · 2015Article
- Electrophoretic mobility of supercoiled, catenated and knotted DNA molecules.Nucleic acids research · 2015Article
- 55.2, a phage T4 ORFan gene, encodes an inhibitor of Escherichia coli topoisomerase I and increases phage fitness.PloS one · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Agarose-gel electrophoresis has been used for more than thirty years to characterize the linking-number (Lk) distribution of closed-circular DNA molecules. Although the physical basis of this technique remains poorly understood, the gel-electrophoretic behavior of covalently closed DNAs has been used to determine the local unwinding of DNA by proteins and small-molecule ligands, characterize supercoiling-dependent conformational transitions in duplex DNA, and to measure helical-repeat changes due to shifts in temperature and ionic strength. Those results have been analyzed by assuming that the absolute mobility of a particular topoisomer is mainly a function of the integral number of superhelical turns, and thus a slowly varying function of plasmid molecular weight. In examining the mobilities of Lk topoisomers for a series of plasmids that differ incrementally in size over more than one helical turn, we found that the size-dependent agarose-gel mobility of individual topoisomers with identical values of Lk (but different values of the excess linking number, DeltaLk) vary dramatically over a duplex turn. Our results suggest that a simple semi-empirical relationship holds between the electrophoretic mobility of linking-number topoisomers and their average writhe in solution.
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