ArticleProteome science2010
Proteome analysis of human Wharton's jelly cells during in vitro expansion.
Article in Proteome science, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed.
- Validated methods for isolation and qualification of mesenchymal stromal/stem cells from different sources.Journal of translational medicine · 2025Review
- Proteomic Characterization of Changes in Mouse Brain Cortex Protein Expression at Different Post-Mortem Intervals: A Preliminary Study for Forensic Biomarker Identification.International journal of molecular sciences · 2024Article
- Efficient improvement of the proliferation, differentiation, and anti-arthritic capacity of mesenchymal stem cells by simply culturing on the immobilized FGF2 derived peptide, 44-ERGVVSIKGV-53.Journal of advanced research · 2024Article
- Article
- The role of the adenylate kinase 5 gene in various diseases and cancer.Journal of clinical and translational science · 2024Review
- Proteomic Signature and mRNA Expression in Hippocampus of SAMP8 and SAMR1 Mice during Aging.International journal of molecular sciences · 2022Article
- Proteomic Characterization of Two Extracellular Vesicle Subtypes Isolated from Human Glioblastoma Stem Cell Secretome by Sequential Centrifugal Ultrafiltration.Biomedicines · 2021Article
- Allogeneic human umbilical cord Wharton's jelly stem cells increase several-fold the expansion of human cord blood CD34+ cells both in vitro and in vivo.Stem cell research & therapy · 2020Article
- Therapeutic uses of post-partum tissue-derived mesenchymal stromal cell secretome.The Indian journal of medical research · 2020Review
- Diameters and Fluorescence Calibration for Extracellular Vesicle Analyses by Flow Cytometry.International journal of molecular sciences · 2020Article
- Current Trends and Future Perspective of Mesenchymal Stem Cells and Exosomes in Corneal Diseases.International journal of molecular sciences · 2019Review
- Manufacturing of primed mesenchymal stromal cells for therapy.Nature biomedical engineering · 2019Review
- Potentiation of temozolomide antitumor effect by purine receptor ligands able to restrain the in vitro growth of human glioblastoma stem cells.Purinergic signalling · 2015Article
- Umbilical Cord Tissue-Derived Cells as Therapeutic Agents.Stem cells international · 2015Review
- Human Mesenchymal Stem Cells Reendothelialize Porcine Heart Valve Scaffolds: Novel Perspectives in Heart Valve Tissue Engineering.BioResearch open access · 2015Article
- Article
- Cryopreservation effects on Wharton's Jelly Stem Cells proteome.Stem cell reviews and reports · 2014Article
- Stem Cell Banking for Regenerative and Personalized Medicine.Biomedicines · 2014Review
- Gene expression modifications in Wharton's Jelly mesenchymal stem cells promoted by prolonged in vitro culturing.BMC genomics · 2013Article
- The therapeutic potential, challenges and future clinical directions of stem cells from the Wharton's jelly of the human umbilical cord.Stem cell reviews and reports · 2013Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe human umbilical cord contains mucoid connective tissue and fibroblast-like cells. These cells named Wharton's jelly cells, (WJCs) display properties similar to mesenchymal stem cells therefore representing a rich source of primitive cells to be potentially used in regenerative medicine.
resultsTo better understand their self-renewal and potential in vitro expansion capacity, a reference 2D map was constructed as a proteomic data set. 158 unique proteins were identified. More than 30% of these proteins belong to cytoskeleton compartment. We also found that several proteins including Shootin1, Adenylate kinase 5 isoenzyme and Plasminogen activator-inhibitor 2 are no longer expressed after the 2nd passage of in vitro replication. This indicates that the proliferative potency of these cells is reduced after the initial stage of in vitro growing. At the end of cellular culturing, new synthesized proteins, including, ERO1-like protein alpha, Aspartyl-tRNA synthetase and Prolyl-4-hydroxylase were identified. It is suggested that these new synthesized proteins are involved in the impairment of cellular surviving during replication and differentiation time.
conclusionsOur work represents an essential step towards gaining knowledge of the molecular properties of WJCs so as to better understand their possible use in the field of cell therapy and regenerative medicine.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.