ArticleBMC cancer2010
Differential profiling of breast cancer plasma proteome by isotope-coded affinity tagging method reveals biotinidase as a breast cancer biomarker.
Article in BMC cancer, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 76 citations in OpenAlex.
- Biotin Homeostasis and Human Disorders: Recent Findings and Perspectives.International journal of molecular sciences · 2024Review
- Comparative profiling by data-independent acquisition mass spectrometry reveals featured plasma proteins in breast cancer: a pilot study.Annals of surgical treatment and research · 2024Article
- Potential therapeutic targets for membranous nephropathy: proteome-wide Mendelian randomization and colocalization analysis.Frontiers in immunology · 2024Article
- Plasma proteome of growing tumors.Scientific reports · 2023Article
- Diagnostic accuracy of a three-protein signature in women with suspicious breast lesions: a multicenter prospective trial.Breast cancer research : BCR · 2023Observational
- Advancements in Oncoproteomics Technologies: Treading toward Translation into Clinical Practice.Proteomes · 2023Review
- Diagnostic performance improvement with combined use of proteomics biomarker assay and breast ultrasound.Breast cancer research and treatment · 2022Article
- Proteomic Interrogation in Cancer Biomarker.Advances in experimental medicine and biology · 2021Article
- StanDep: Capturing transcriptomic variability improves context-specific metabolic models.PLoS computational biology · 2020Article
- Hippocampal proteomic changes of susceptibility and resilience to depression or anxiety in a rat model of chronic mild stress.Translational psychiatry · 2019Article
- Integrative machine learning analysis of multiple gene expression profiles in cervical cancer.PeerJ · 2018Article
- Advancement of mass spectrometry-based proteomics technologies to explore triple negative breast cancer.Molecular bioSystems · 2016Review
- Exome genotyping arrays to identify rare and low frequency variants associated with epithelial ovarian cancer risk.Human molecular genetics · 2016Article
- Quantitative Proteomic Analysis Reveals Molecular Adaptations in the Hippocampal Synaptic Active Zone of Chronic Mild Stress-Unsusceptible Rats.The international journal of neuropsychopharmacology · 2015Article
- Biotin starvation causes mitochondrial protein hyperacetylation and partial rescue by the SIRT3-like deacetylase Hst4p.Nature communications · 2015Article
- Quantitative variability of 342 plasma proteins in a human twin population.Molecular systems biology · 2015Article
- Proteomic approaches in biomarker discovery: new perspectives in cancer diagnostics.TheScientificWorldJournal · 2014Review
- Comparative profiling of plasma proteome from breast cancer patients reveals thrombospondin-1 and BRWD3 as serological biomarkers.Experimental & molecular medicine · 2012Article
- Article
- Proteomic technologies for the study of osteosarcoma.Sarcoma · 2012Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBreast cancer is one of the leading causes of women's death worldwide. It is important to discover a reliable biomarker for the detection of breast cancer. Plasma is the most ideal source for cancer biomarker discovery since many cells cross-communicate through the secretion of soluble proteins into blood.
methodsPlasma proteomes obtained from 6 breast cancer patients and 6 normal healthy women were analyzed by using the isotope-coded affinity tag (ICAT) labeling approach and tandem mass spectrometry. All the plasma samples used were depleted of highly abundant 6 plasma proteins by immune-affinity column chromatography before ICAT labeling. Several proteins showing differential abundance level were selected based on literature searches and their specificity to the commercially available antibodies, and then verified by immunoblot assays.
resultsA total of 155 proteins were identified and quantified by ICAT method. Among them, 33 proteins showed abundance changes by more than 1.5-fold between the plasmas of breast cancer patients and healthy women. We chose 5 proteins for the follow-up confirmation in the individual plasma samples using immunoblot assay. Four proteins, alpha1-acid glycoprotein 2, monocyte differentiation antigen CD14, biotinidase (BTD), and glutathione peroxidase 3, showed similar abundance ratio to ICAT result. Using a blind set of plasmas obtained from 21 breast cancer patients and 21 normal healthy controls, we confirmed that BTD was significantly down-regulated in breast cancer plasma (Wilcoxon rank-sum test, p = 0.002). BTD levels were lowered in all cancer grades (I-IV) except cancer grade zero. The area under the receiver operating characteristic curve of BTD was 0.78. Estrogen receptor status (p = 0.940) and progesterone receptor status (p = 0.440) were not associated with the plasma BTD levels.
conclusionsOur study suggests that BTD is a potential serological biomarker for the detection of breast cancer.
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