ArticleThe FEBS journal2010
A new bright green-emitting fluorescent protein--engineered monomeric and dimeric forms.
Article in The FEBS journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed.
- Fluorescence Guided Raman Spectroscopy enables the training of robust support vector machines for the detection of tumour marker proteins.Scientific reports · 2025Article
- Spatiotemporal control of cell ablation using Ronidazole with Nitroreductase in Drosophila.Developmental biology · 2025Article
- Imaging different cell populations in the mouse olfactory bulb using the genetically encoded voltage indicator ArcLight.Neurophotonics · 2024Review
- Ultrafast fluorescence depolarisation in green fluorescence protein tandem dimers as hydrophobic environment sensitive probes.Physical chemistry chemical physics : PCCP · 2023Article
- Skin Immuno-CometChip in 3D vs. 2D Cultures to Screen Topical Toxins and Skin-Specific Cytochrome Inducers.Genes · 2023Article
- Production and applications of fluorobody from redox-engineered Escherichia coli.Applied microbiology and biotechnology · 2023Article
- Nr4a1 and Nr4a3 Reporter Mice Are Differentially Sensitive to T Cell Receptor Signal Strength and Duration.Cell reports · 2020Article
- Multiplex T Cell Stimulation Assay Utilizing a T Cell Activation Reporter-Based Detection System.Frontiers in immunology · 2020Article
- Molecular evolution of versatile derivatives from a GFP-like protein in the marine copepod Chiridius poppei.PloS one · 2017Article
- The cytoplasmic domain is essential for transport function of the integral membrane transport protein SLC4A11.American journal of physiology. Cell physiology · 2016Article
- Improving recombinant protein production in the Chlamydomonas reinhardtii chloroplast using vivid Verde Fluorescent Protein as a reporter.Biotechnology journal · 2015Article
- Protein design: Past, present, and future.Biopolymers · 2015Review
- Spectral and structural comparison between bright and dim green fluorescent proteins in Amphioxus.Scientific reports · 2014Article
- Transcriptome deep-sequencing and clustering of expressed isoforms from Favia corals.BMC genomics · 2013Article
- Article
- Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%.Nature communications · 2012Article
- Water Diffusion In And Out Of The β-Barrel Of GFP and The Fast Maturing Fluorescent Protein, TurboGFP.Chemical physics · 2012Article
- Stimuli-responsive smart gels realized via modular protein design.Journal of the American Chemical Society · 2010Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Fluorescent proteins have become essential tools in molecular and biological applications. Here, we present a novel fluorescent protein isolated from warm water coral, Cyphastrea microphthalma. The protein, which we named vivid Verde fluorescent protein (VFP), matures readily at 37 degrees C and emits bright green light. Further characterizations revealed that VFP has a tendency to form dimers. By creating a homology model of VFP, based on the structure of the red fluorescent protein, DsRed, we were able to make mutations that alter the protein's oligomerization state. We present two proteins, mVFP and mVFP1, that are both exclusively monomeric, and one protein, dVFP, which is dimeric. We characterized the spectroscopic properties of VFP and its variants in comparison with enhanced green fluorescent protein (EGFP), a widely used variant of GFP. All the VFP variants are at least twice as bright as EGFP. Finally, we demonstrated the effectiveness of the VFP variants in both in vitro and in vivo detection applications.
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Registered trials
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