ArticleMolecular microbiology2010
Pleiotropic function of intersectin homologue Cin1 in Cryptococcus neoformans.
Article in Molecular microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 30 citations in OpenAlex.
- Genetic Transformation inJournal of fungi (Basel, Switzerland) · 2021Review
- Pleiotropy and epistasis within and between signaling pathways defines the genetic architecture of fungal virulence.PLoS genetics · 2021Article
- Pathogenic Delivery: The Biological Roles of Cryptococcal Extracellular Vesicles.Pathogens (Basel, Switzerland) · 2020Review
- Transcriptomic Analysis of Extracellular RNA Governed by the Endocytic Adaptor Protein Cin1 ofFrontiers in cellular and infection microbiology · 2020Article
- The TOR Pathway Plays Pleiotropic Roles in Growth and Stress Responses of the Fungal PathogenGenetics · 2019Article
- The cAMP/Protein Kinase a Pathway Regulates Virulence and Adaptation to Host Conditions inFrontiers in cellular and infection microbiology · 2019Review
- MoCAP proteins regulated by MoArk1-mediated phosphorylation coordinate endocytosis and actin dynamics to govern development and virulence of Magnaporthe oryzae.PLoS genetics · 2017Article
- Comparative Ecology of CapsularFrontiers in microbiology · 2017Article
- Intersectin scaffold proteins and their role in cell signaling and endocytosis.Biochimica et biophysica acta. Molecular cell research · 2017Review
- Comparative proteomic analysis of Gib2 validating its adaptor function in Cryptococcus neoformans.PloS one · 2017Article
- Intron retention-dependent gene regulation in Cryptococcus neoformans.Scientific reports · 2016Article
- A Ric8/synembryn homolog promotes Gpa1 and Gpa2 activation to respectively regulate cyclic AMP and pheromone signaling in Cryptococcus neoformans.Eukaryotic cell · 2014Article
- Noncanonical Gβ Gib2 is a scaffolding protein promoting cAMP signaling through functions of Ras1 and Cac1 proteins in Cryptococcus neoformans.The Journal of biological chemistry · 2014Article
- Fungal alternative splicing is associated with multicellular complexity and virulence: a genome-wide multi-species study.DNA research : an international journal for rapid publication of reports on genes and genomes · 2014Article
- The actin-regulating kinase homologue MoArk1 plays a pleiotropic function in Magnaporthe oryzae.Molecular plant pathology · 2013Article
- Emerging roles for intersectin (ITSN) in regulating signaling and disease pathways.International journal of molecular sciences · 2013Review
- Aimless mutants of Cryptococcus neoformans: failure to disseminate.Fungal biology reviews · 2012Article
- The Cryptococcus neoformans capsule: a sword and a shield.Clinical microbiology reviews · 2012Review
- Wsp1 is downstream of Cin1 and regulates vesicle transport and actin cytoskeleton as an effector of Cdc42 and Rac1 in Cryptococcus neoformans.Eukaryotic cell · 2012Article
- The endocytic adaptor proteins of pathogenic fungi: charting new and familiar pathways.Medical mycology · 2011Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The manifestation of virulence traits in Cryptococcus neoformans is thought to rely on intracellular transport, a process not fully explored in this pathogenic fungus. Through interaction cloning, we identified a multi-modular protein, Cin1 (cryptococcal intersectin 1), whose domain structure is similar to that of the human endocytic protein ITSN1. Cin1 contains an N-terminal EH domain, a central coiled-coil region, a WH2 domain, two SH3 domains and a C-terminal RhoGEF (DH)-PH domain. Interestingly, alternative mRNA splicing resulted in two Cin1 isoforms, and Cin1 homologues are also restricted to basidiomycetous fungi. Disruption of the CIN1 gene had a pleiotropic effect on growth, normal cytokinesis, intracellular transports and the production of several virulence factors. Additionally, Cin1 interacts with cryptococcal Cdc42 and Wsp1 (a WASP homologue) proteins in vitro, suggesting a conserved role in the regulation of the actin cytoskeleton. However, deletion of RhoGEF or SH3 and RhoGEF domains did not result in any phenotypic changes, suggesting that functional redundancy exists in proteins containing similar domains or that the activities by other domains are necessary for Cin1 function. Our study presents the first evidence of a multi-modular protein whose function in intracellular transport underlies the growth, differentiation and virulence of a pathogenic microorganism.
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