ArticleMolecular microbiology2010
CobB regulates Escherichia coli chemotaxis by deacetylating the response regulator CheY.
Article in Molecular microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
48 citing papers in PubMed, 87 citations in OpenAlex.
- Characterization of the Nicotinamide Adenine Dinucleotide Biosynthesis Pathway and Regulatory Mechanisms in Streptococcus mutans.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Article
- Intracellular acetyl phosphate modulatesActa biochimica et biophysica Sinica · 2025Article
- Microbiota-derived succinate promotes enterohaemorrhagic Escherichia coli virulence via lysine succinylation.Nature microbiology · 2025Article
- Review
- An insight into the role of the N-terminal domain ofFrontiers in molecular biosciences · 2024Article
- Systematic Analysis of Lysine Acetylation Reveals Diverse Functions in Azorhizobium caulinodans Strain ORS571.Microbiology spectrum · 2023Article
- Apprehending the NADViruses · 2022Article
- Methylation of PhoP by CheR RegulatesmBio · 2021Article
- Article
- The switching mechanism of the bacterial rotary motor combines tight regulation with inherent flexibility.The EMBO journal · 2021Article
- Genome-Wide Analyses of Proteome and Acetylome inFrontiers in microbiology · 2021Article
- Bacterial Sirtuins Overview: An Open Niche to Explore.Frontiers in microbiology · 2021Review
- Lysine acetylation of the housekeeping sigma factor enhances the activity of the RNA polymerase holoenzyme.Nucleic acids research · 2020Article
- A single regulator NrtR controls bacterial NADeLife · 2019Article
- Interplay between the bacterial protein deacetylase CobB and the second messenger c-di-GMP.The EMBO journal · 2019Article
- Protein Acetylation in Bacteria.Annual review of microbiology · 2019Review
- Metabolic intermediate acetyl phosphate modulates bacterial virulence via acetylation.Emerging microbes & infections · 2019Article
- Post-translational Protein Acetylation: An Elegant Mechanism for Bacteria to Dynamically Regulate Metabolic Functions.Frontiers in microbiology · 2019Review
- Acetylation of lysine 182 inhibits the ability of Mycobacterium tuberculosis DosR to bind DNA and regulate gene expression during hypoxia.Emerging microbes & infections · 2018Article
- Protein Acetylation Mediated by YfiQ and CobB Is Involved in the Virulence and Stress Response of Yersinia pestis.Infection and immunity · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The silent information regulator (Sir2) family proteins are NAD+-dependent deacetylases. Although a few substrates have been identified, functions of the bacteria Sir2-like protein (CobB) still remain unclear. Here the role of CobB on Escherichia coli chemotaxis was investigated. We used Western blotting and mass spectrometry to show that the response regulator CheY is a substrate of CobB. Surface plasmon resonance (SPR) indicated that acetylation affects the interaction between CheY and the flagellar switch protein FliM. The presence of intact flagella in knockout strains DeltacobB, Deltaacs, Delta(cobB) Delta(acs), Delta(cheA) Delta(cheZ), Delta(cheA) Delta(cheZ) Delta(cobB) and Delta(cheA) Delta(cheZ) Delta(acs) was confirmed by electron microscopy. Genetic analysis of these knockout strains showed that: (i) the DeltacobB mutant exhibited reduced responses to chemotactic stimuli in chemotactic assays, whereas the Deltaacs mutant was indistinguishable from the parental strain, (ii) CheY from the DeltacobB mutant showed a higher level of acetylation, indicating that CobB can mediate the deacetylation of CheY in vivo, and (iii) deletion of cobB reversed the phenotype of Delta(cheA) Delta(cheZ). Our findings suggest that CobB regulates E. coli chemotaxis by deacetylating CheY. Thus a new function of bacterial cobB was identified and also new insights of regulation of bacterial chemotaxis were provided.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.