ArticleMolecular microbiology2010
Shear-enhanced binding of intestinal colonization factor antigen I of enterotoxigenic Escherichia coli.
Article in Molecular microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Review
- Effect of Gravity on Bacterial Adhesion to Heterogeneous Surfaces.Pathogens (Basel, Switzerland) · 2023Article
- Neutralizing Antibodies Against Allosteric Proteins: Insights From a Bacterial Adhesin.Journal of molecular biology · 2022Review
- Recombinant FimH Adhesin Demonstrates How the Allosteric Catch Bond Mechanism Can Support Fast and Strong Bacterial Attachment in the Absence of Shear.Journal of molecular biology · 2022Article
- Mechanical Forces Govern Interactions of Host Cells with Intracellular Bacterial Pathogens.Microbiology and molecular biology reviews : MMBR · 2022Review
- Cross-Reactivity, Epitope Mapping, and Potency of Monoclonal Antibodies to Class 5 Fimbrial Tip Adhesins of Enterotoxigenic Escherichia coli.Infection and immunity · 2020Article
- Mechanical Properties and Concentrations of Poly(ethylene glycol) in Hydrogels and Brushes Direct the Surface Transport of Staphylococcus aureus.ACS applied materials & interfaces · 2019Article
- Adhesion of the genome-sequenced Lactococcus lactis subsp. cremoris IBB477 strain is mediated by specific molecular determinants.Applied microbiology and biotechnology · 2016Article
- Mechanism of allosteric propagation across a β-sheet structure investigated by molecular dynamics simulations.Proteins · 2016Article
- Binding of CFA/I Pili of Enterotoxigenic Escherichia coli to Asialo-GM1 Is Mediated by the Minor Pilin CfaE.Infection and immunity · 2016Article
- The mechanical world of bacteria.Cell · 2015Review
- Inhibition and Reversal of Microbial Attachment by an Antibody with Parasteric Activity against the FimH Adhesin of Uropathogenic E. coli.PLoS pathogens · 2015Article
- Host attachment and fluid shear are integrated into a mechanical signal regulating virulence in Escherichia coli O157:H7.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Structural and functional insight into the carbohydrate receptor binding of F4 fimbriae-producing enterotoxigenic Escherichia coli.The Journal of biological chemistry · 2015Article
- Regulatory T-cell vaccination independent of auto-antigen.Experimental & molecular medicine · 2014Review
- Between Amyloids and Aggregation Lies a Connection with Strength and Adhesion.New journal of science · 2014Article
- Structural and population characterization of MrkD, the adhesive subunit of type 3 fimbriae.Journal of bacteriology · 2013Article
- Allosteric coupling in the bacterial adhesive protein FimH.The Journal of biological chemistry · 2013Article
- Tight conformational coupling between the domains of the enterotoxigenic Escherichia coli fimbrial adhesin CfaE regulates binding state transition.The Journal of biological chemistry · 2013Article
- Article
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7 authors.
Funding
Abstract
In the intestine, enterotoxigenic Escherichia coli works against peristaltic forces, adhering to the epithelium via the colonization factor antigen I (CFA/I) fimbrial adhesin CfaE. The CfaE adhesin is similar in localization and tertiary (but not primary) structure to FimH, the type 1 fimbrial adhesin of uropathogenic E. coli, which shows shear-dependent binding to epithelial receptors by an allosteric catch-bond mechanism. Thus, we speculated that CfaE is also capable of shear-enhanced binding. Indeed, bovine erythrocytes coursing over immobilized CFA/I fimbriae in flow chambers exhibited low accumulation levels and fast rolling at low shear, but an 80-fold increase in accumulation and threefold decrease in rolling velocity at elevated shear. This effect was reversible and abolished by pre-incubation of fimbriae with anti-CfaE antibody. Erythrocytes bound to whole CfaE in the same shear-enhanced manner, but to CfaE adhesin domain in a shear-inhibitable fashion. Residue replacements designed to disrupt CfaE interdomain interaction decreased the shear dependency of adhesion and increased binding under static conditions to human intestinal epithelial cells. These findings indicate that close interaction between adhesive and anchoring pilin domains of CfaE keeps the former in a low-affinity state that toggles into a high-affinity state upon separation of two domains, all consistent with an allosteric catch-bond mechanism of CfaE binding.
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