ArticleMolecular biotechnology2010
Reference gene selection for real-time RT-PCR in eight kinds of rat regenerating hepatic cells.
Article in Molecular biotechnology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Article
- Identification of stable housekeeping genes in mouse liver for studying carbon tetrachloride-induced injury and cellular senescence.Scientific reports · 2024Article
- Comparative analysis of the expression profiles of genes related to the Gadd45α signaling pathway in four kinds of liver diseases.Histology and histopathology · 2020Article
- Purinergic signaling in hepatic disease.Purinergic signalling · 2019Review
- Article
- A preliminary in vivo study of the effects of OPN on rat liver regeneration induced by partial hepatectomy.Molecular biology reports · 2016Article
- Comparative analysis of gene expression profiles of OPN signaling pathway in four kinds of liver diseases.Journal of genetics · 2016Review
- Analysis of the ways and methods of signaling pathways in regulating cell cycle of NIH3T3 at transcriptional level.BMC cell biology · 2015Article
- Screening for Suitable Reference Genes for Quantitative Real-Time PCR in Heterosigma akashiwo (Raphidophyceae).PloS one · 2015Article
- Transcription Profiles of Marker Genes Predict The Transdifferentiation Relationship between Eight Types of Liver Cell during Rat Liver Regeneration.Cell journal · 2015Article
- Real-time qPCR identifies suitable reference genes for Borna disease virus-infected rat cortical neurons.International journal of molecular sciences · 2014Article
- Expression profiles uncover the relationship between erythropoietin and cell proliferation in rat hepatocytes after a partial hepatectomy.Cellular & molecular biology letters · 2014Article
- Study on the activity of the signaling pathways regulating hepatocytes from G0 phase into G1 phase during rat liver regeneration.Cellular & molecular biology letters · 2014Article
- Article
- Reference gene screening for analyzing gene expression across goat tissue.Asian-Australasian journal of animal sciences · 2013Article
- Bicarbonate-sensitive soluble and transmembrane adenylyl cyclases in peripheral chemoreceptors.Respiratory physiology & neurobiology · 2013Article
- Defining suitable reference genes for RT-qPCR analysis on intestinal epithelial cells.Molecular biotechnology · 2013Article
- Histological evidence: housekeeping genes beta-actin and GAPDH are of limited value for normalization of gene expression.Development genes and evolution · 2012Article
- Analysis of the role of the integrin signaling pathway in hepatocytes during rat liver regeneration.Cellular & molecular biology letters · 2012Article
- Effect of hyperoxic exposure during early development on neurotrophin expression in the carotid body and nucleus tractus solitarii.Journal of applied physiology (Bethesda, Md. : 1985) · 2012Article
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2 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Liver regeneration (LR) is a process during which the liver recovers its mass and function after damage due to various causes such as partial hepatectomy (PH). It involves a sequence of well-orchestrated changes in physiological and biochemical activities, especially in the gene expression profile in a variety of liver cells. In order to produce reliable gene expression of target genes in eight kinds of rat hepatic cells during LR, the determination of internal control housekeeping genes (HKGs) is required. Eight kinds of hepatic cells were first isolated from liver tissue with high purity and activity. Then quantitative real-time reverse transcription (RT)-PCR was applied to detect expression changes of six commonly used HKGs (18SrRNA, B2M, ACTB, UBC, GAPDH, and HK1) in eight types of hepatic cells isolated from regenerating liver at 0, 2, 6, 12, 24, 30, 36, 72, 120, and 168 h after PH. The amplification of the HKGs was statistically analyzed by using geNorm algorithm. Using this method, 18SrRNA-UBC, ACTB-HK1, ACTB-GADPH, B2M-ACTB, 18SrRNA-UBC, B2M-UBC, B2M-ACTB, and B2M-UBC were found to be the two most stable reference genes for rat regenerating hepatocytes, hepatic stellate cells, Kupffer cells, biliary epithelial cells, sinusoidal endothelial cells, pit cells, dendritic cells, and oval cells, respectively, regardless of the stages of LR. In conclusion, this study has laid a good foundation for investigating gene expression of target genes in different types of hepatic cells during LR.
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