ArticleCellular and molecular life sciences : CMLS2010
Vector-based RNA interference of cathepsin B1 in Schistosoma mansoni.
Article in Cellular and molecular life sciences : CMLS, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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Who cites it
25 citing papers in PubMed, 44 citations in OpenAlex.
- Schistosome Transgenesis: The Long Road to Success.Biology · 2024Review
- A low dose adenovirus vectored vaccine expressing Schistosoma mansoni Cathepsin B protects from intestinal schistosomiasis in mice.EBioMedicine · 2022Article
- Developmental Sensitivity in Schistosoma mansoni to Puromycin To Establish Drug Selection of Transgenic Schistosomes.Antimicrobial agents and chemotherapy · 2018Article
- RNA interference in Fasciola gigantica: Establishing and optimization of experimental RNAi in the newly excysted juveniles of the fluke.PLoS neglected tropical diseases · 2017Article
- Identification and validation of a Schistosoma japonicum U6 promoter.Parasites & vectors · 2017Article
- New research tools for urogenital schistosomiasis.The Journal of infectious diseases · 2015Review
- Omega-1 knockdown in Schistosoma mansoni eggs by lentivirus transduction reduces granuloma size in vivo.Nature communications · 2014Article
- Expression profile of the Schistosoma japonicum degradome reveals differential protease expression patterns and potential anti-schistosomal intervention targets.PLoS computational biology · 2014Article
- RNAi dynamics in Juvenile Fasciola spp. Liver flukes reveals the persistence of gene silencing in vitro.PLoS neglected tropical diseases · 2014Article
- Pseudotyped murine leukemia virus for schistosome transgenesis: approaches, methods and perspectives.Transgenic research · 2014Article
- Article
- Review
- Polyethyleneimine mediated DNA transfection in schistosome parasites and regulation of the WNT signaling pathway by a dominant-negative SmMef2.PLoS neglected tropical diseases · 2013Article
- Applications of RNA Interference in Schistosomiasis: Gene Function Identification and Development of New Therapies.ISRN parasitology · 2013Review
- Human U6 promoter drives stronger shRNA activity than its schistosome orthologue in Schistosoma mansoni and human fibrosarcoma cells.Transgenic research · 2012Article
- Prototypic chromatin insulator cHS4 protects retroviral transgene from silencing in Schistosoma mansoni.Transgenic research · 2012Article
- Biogenic amines and the control of neuromuscular signaling in schistosomes.Invertebrate neuroscience : IN · 2012Review
- Nucleic acid transfection and transgenesis in parasitic nematodes.Parasitology · 2012Review
- Genetic manipulation of schistosomes--progress with integration competent vectors.Parasitology · 2012Review
- Germline transgenesis and insertional mutagenesis in Schistosoma mansoni mediated by murine leukemia virus.PLoS pathogens · 2012Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
In helminth parasites, proteolytic enzymes have been implicated in facilitating host invasion, moulting, feeding, and evasion of the host immune response. These key functions render them potential targets for anti-parasite chemotherapy and immunotherapy. Schistosomes feed on host blood and the digested haemoglobin is their major source of amino acids. Haemoglobin digestion is essential for parasite development, growth, and reproduction. We recently reported the use of pseudotyped Moloney murine leukaemia virus to accomplish transformation of Schistosoma mansoni. Here, we report the design of a viral vector expressing a dsRNA hairpin to silence expression of the schistosome cathepsin B1 (SmCB1) gene. We observed 80% reduction in transcript level 72 h after virus exposure and complete silencing of enzyme activity in transduced worms. This is the first report using this technology in any helminth parasite. It will facilitate the evaluation of potential drug targets and biochemical pathways for novel interventions in schistosomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.