ArticleJournal of biomedicine & biotechnology2010
Characterization of recombinant dengue-2 virus derived from a single nucleotide substitution in the 5' noncoding region.
Article in Journal of biomedicine & biotechnology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Therapeutic Potential of Medicinal Plants against Dengue Infection: A Mechanistic Viewpoint.ACS omega · 2022Review
- Molecular Characterization and Phylogenetic Analysis of the 2019 Dengue Outbreak in Wenzhou, China.Frontiers in cellular and infection microbiology · 2022Article
- Full-length genome and molecular characterization of dengue virus serotype 2 isolated from an imported patient from Myanmar.Virology journal · 2018Article
- Flavivirus reverse genetic systems, construction techniques and applications: a historical perspective.Antiviral research · 2015Review
- Comparison of full-length genomics sequences between dengue virus serotype 3, parental strain, and its derivatives, and B-cell epitopes prediction from envelope region.Bioinformation · 2013Article
- Development of Dengue type-2 virus replicons expressing GFP reporter gene in study of viral RNA replication.Virus research · 2012Article
- A single amino acid in nonstructural protein NS4B confers virulence to dengue virus in AG129 mice through enhancement of viral RNA synthesis.Journal of virology · 2011Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Variants of wild-type dengue serotype 2 (DEN-2) virus containing nucleotide substitutions at positions 14, 15, or 57 in the 5' NCR were constructed by PCR-mediated site-directed mutagenesis. All three viruses containing a single point substitution demonstrated attenuation phenotype as evidenced by decreases replication and plaque size in cell culture assay. All three variants were less neurovirulent in newborn mice compared to the wild type. The mutants were immunogenic in adult mice immunogenicity and maintained stable replication characteristics following passage in mice. The variant viruses were competent for replication in Aedes aegypi mosquito vector, albeit at lower levels of infection and dissemination in the mosquito than the wild-type Den-2 16681 virus. Although all of the viruses, including the wild type, were found transmissible in mosquito life cycles, they were found subsequentially decreased in efficiency of infection, transmission, and dissemination rates along the mosquito generations and all of them remained genetically stable.
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