ArticleJournal of insect science (Online)2007
Immunogold localization of vitellogenin in the ovaries, hypopharyngeal glands and head fat bodies of honeybee workers, Apis mellifera.
Article in Journal of insect science (Online), 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed, 84 citations in OpenAlex.
- AI-enhanced marker-assisted selection concept for the multifunctional honey bee (Hymenoptera: Apidea) protein Vitellogenin (Vg).Journal of economic entomology · 2025Article
- Review
- Effects of Pollen Deprivation in Groups of Tellian (Insects · 2022Article
- Single-cell transcriptomic analysis of honeybee brains identifies vitellogenin as caste differentiation-related factor.iScience · 2022Article
- Differential Expression of Major Royal Jelly Proteins in the Hypopharyngeal Glands of the HoneybeeInsects · 2022Article
- Nuclear translocation of vitellogenin in the honey bee (Apidologie · 2022Article
- Changes in gut microbiota and metabolism associated with phenotypic plasticity in the honey beeFrontiers in microbiology · 2022Article
- Gut-Expressed Vitellogenin Facilitates the Movement of a Plant Virus across the Midgut Wall in Its Insect Vector.mSystems · 2021Article
- A field-based quantitative analysis of sublethal effects of air pollution on pollinators.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- The Year of the Honey Bee (Insects · 2019Article
- Vitellogenin transcytosis in follicular cells of the honeybee Apis mellifera and the wasp Polistes simillimus.Protoplasma · 2018Article
- Transcriptional Control of Honey Bee (Insects · 2018Article
- The transcriptomic changes associated with the development of social parasitism in the honeybee Apis mellifera capensis.Die Naturwissenschaften · 2018Article
- Insect tissue-specific vitellogenin facilitates transmission of plant virus.PLoS pathogens · 2018Article
- Article
- Persistence of subclinical deformed wing virus infections in honeybees following Varroa mite removal and a bee population turnover.PloS one · 2017Article
- Vitellogenin knockdown strongly affects cotton boll weevil egg viability but not the number of eggs laid by females.Meta gene · 2016Article
- Changes in follicular cells architecture during vitellogenin transport in the ovary of social Hymenoptera.Protoplasma · 2016Article
- Article
- Gene Expression Dynamics in Major Endocrine Regulatory Pathways along the Transition from Solitary to Social Life in a Bumblebee,Frontiers in physiology · 2016Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Vitellogenin is a yolk precursor protein in most oviparous females. In the advanced eusocial honeybee, Apis mellifera (Hymenoptera: Apidae), vitellogenin has recently attracted much interest as this protein, in addition to a classical function in oocyte development in the reproductive queen caste, has evolved functions in the facultatively sterile female worker caste not documented in other species. However, research on the spatial dynamics of vitellogenin in various tissues is not easily performed with available tools. Here we present an immunogold staining procedure that visualizes honeybee vitellogenin in resin embedded tissue. To establish the protocol, we used ovaries of worker bees from colonies with and without a queen. Under the first condition, vitellogenin is assumed not to be present in the workers' ovaries. Under the second condition, the ovaries of worker bees become vitellogenic, with abundant opportunities for detection of complex patterns of vitellogenin uptake and storage. By use of this experimental setup, the staining method is shown to be both sensitive and specific. To demonstrate the functional significance of the protocol, it was subsequently used to identify vitellogenin protein in the hypopharyngeal glands (brood food producing head glands) of nursing worker bees and in adjacent head fat body cells for the first time. Localization of vitellogenin in these tissues supports previously hypothesized roles of vitellogenin in social behavior. This protocol thus provides deeper insights into the functions of vitellogenin in the honeybee.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.