ArticleTissue engineering. Part C, Methods2010
Neuromuscular junction formation between human stem-cell-derived motoneurons and rat skeletal muscle in a defined system.
Article in Tissue engineering. Part C, Methods, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 70 citations in OpenAlex.
- Unlocking the Complexity of Neuromuscular Diseases: Insights from Human Pluripotent Stem Cell-Derived Neuromuscular Junctions.International journal of molecular sciences · 2023Review
- The Effect of Skeletal Muscle-Specific Creatine Treatment on ALS NMJ Integrity and Function.International journal of molecular sciences · 2023Article
- Development of a human malaria-on-a-chip disease model for drug efficacy and off-target toxicity evaluation.Scientific reports · 2023Article
- Optimization of Application-Driven Development ofTissue engineering. Part B, Reviews · 2022Review
- Creating stem cell-derived neuromuscular junctions in vitro.Muscle & nerve · 2021Review
- Axonal regeneration and sprouting as a potential therapeutic target for nervous system disorders.Neural regeneration research · 2021Review
- Tissue-Engineered Skeletal Muscle Models to Study Muscle Function, Plasticity, and Disease.Frontiers in physiology · 2021Review
- Organ on a Chip: A NovelFrontiers in neurology · 2021Review
- A Chemically Defined Common Medium for Culture of C2C12 Skeletal Muscle and Human Induced Pluripotent Stem Cell Derived Spinal Spheroids.Cellular and molecular bioengineering · 2020Article
- A Human-Based Functional NMJ System for Personalized ALS Modeling and Drug Testing.Advanced therapeutics · 2020Article
- A Novel Bioengineered Functional Motor Unit Platform to Study Neuromuscular Interaction.Journal of clinical medicine · 2020Article
- In vitro models of neuromuscular junctions and their potential for novel drug discovery and development.Expert opinion on drug discovery · 2020Review
- A multiplexed in vitro assay system for evaluating human skeletal muscle functionality in response to drug treatment.Biotechnology and bioengineering · 2020Article
- Engineered skeletal muscles for disease modeling and drug discovery.Biomaterials · 2019Review
- Toward Building the Neuromuscular Junction: In Vitro Models To Study Synaptogenesis and Neurodegeneration.ACS omega · 2019Review
- Review
- Stem cell derived phenotypic human neuromuscular junction model for dose response evaluation of therapeutics.Biomaterials · 2018Article
- Limitations and Challenges in Modeling Diseases Involving Spinal Motor Neuron DegenerationFrontiers in cellular neuroscience · 2018Article
- A functional human motor unit platform engineered from human embryonic stem cells and immortalized skeletal myoblasts.Stem cells and cloning : advances and applications · 2018Article
- Human-Derived Organ-on-a-Chip for Personalized Drug Development.Current pharmaceutical design · 2018Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
To date, the coculture of motoneurons (MNs) and skeletal muscle in a defined in vitro system has only been described in one study and that was between rat MNs and rat skeletal muscle. No in vitro studies have demonstrated human MN to rat muscle synapse formation, although numerous studies have attempted to implant human stem cells into rat models to determine if they could be of therapeutic use in disease or spinal injury models, although with little evidence of neuromuscular junction (NMJ) formation. In this report, MNs differentiated from human spinal cord stem cells, together with rat skeletal myotubes, were used to build a coculture system to demonstrate that NMJ formation between human MNs and rat skeletal muscles is possible. The culture was characterized by morphology, immunocytochemistry, and electrophysiology, while NMJ formation was demonstrated by immunocytochemistry and videography. This defined system provides a highly controlled reproducible model for studying the formation, regulation, maintenance, and repair of NMJs. The in vitro coculture system developed here will be an important model system to study NMJ development, the physiological and functional mechanism of synaptic transmission, and NMJ- or synapse-related disorders such as amyotrophic lateral sclerosis, as well as for drug screening and therapy design.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.