ArticleJournal of virology1991
Cowpea mosaic virus middle component RNA contains a sequence that allows internal binding of ribosomes and that requires eukaryotic initiation factor 4F for optimal translation.
Article in Journal of virology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 80 citations in OpenAlex.
- Broadening Virus Resistance Through Gene Pyramiding of eIF4E Family Members.Molecular plant pathology · 2025Article
- Article
- Searching for IRES.RNA (New York, N.Y.) · 2006Review
- Cap-independent translation of plant viral RNAs.Virus research · 2006Review
- Polypurine (A)-rich sequences promote cross-kingdom conservation of internal ribosome entry.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Design of multistable RNA molecules.RNA (New York, N.Y.) · 2001Article
- A tertiary structure model of the internal ribosome entry site (IRES) for methionine-independent initiation of translation.RNA (New York, N.Y.) · 2001Article
- Identification and characterization of the functional elements within the tobacco etch virus 5' leader required for cap-independent translation.Journal of virology · 1999Article
- Cleavage of eukaryotic translation initiation factor 4G by exogenously added hybrid proteins containing poliovirus 2Apro in HeLa cells: effects on gene expression.Molecular and cellular biology · 1999Article
- Translation initiation at the CUU codon is mediated by the internal ribosome entry site of an insect picorna-like virus in vitro.Journal of virology · 1999Article
- Gene expression from viral RNA genomes.Plant molecular biology · 1996Review
- Translational control of cellular and viral mRNAs.Plant molecular biology · 1996Review
- Translation in plants--rules and exceptions.Plant molecular biology · 1996Review
- The C-terminal domain of eukaryotic protein synthesis initiation factor (eIF) 4G is sufficient to support cap-independent translation in the absence of eIF4E.The EMBO journal · 1996Article
- The human immunodeficiency virus type 1 5' packaging signal structure affects translation but does not function as an internal ribosome entry site structure.Journal of virology · 1996Article
- Monensin and nigericin prevent the inhibition of host translation by poliovirus, without affecting p220 cleavage.Journal of virology · 1995Article
- An internal ribosomal entry signal in the rat VL30 region of the Harvey murine sarcoma virus leader and its use in dicistronic retroviral vectors.Journal of virology · 1995Article
- Structural analyses of the Epstein-Barr virus BamHI A transcripts.Journal of virology · 1995Article
- Alternative translation of human fibroblast growth factor 2 mRNA occurs by internal entry of ribosomes.Molecular and cellular biology · 1995Article
- Picornavirus inhibitors.Pharmacology & therapeutics · 1994Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cowpea mosaic virus (CPMV) middle component RNA (M-RNA) encodes two proteins of 105 and 95 kDa, of which translation starts at nucleotide (nt) 161 and nt 512, respectively. In vitro translation of both proteins directed by T7 transcripts of M-RNA was stimulated fourfold by eukaryotic initiation factor 4F (eIF-4F), the cap-binding protein complex. The ratio of the synthesis of both proteins after translation was not influenced by eIF-4F or by any known eIF. Part of the CPMV 5' sequence was cloned downstream of the 5' untranslated region of ornithine decarboxylase (ODC); the latter untranslated sequence has a highly stable secondary structure, preventing efficient translation of ODC. Insertion of nt 161 to 512 of CPMV M-RNA upstream of the ODC initiation codon resulted in a marked increase in ODC translation, which indicates that the CPMV sequence contains an internal ribosome-binding site. The insertion conferred stimulation by eIF-4F on ODC translation, showing that eIF-4F is able to stimulate internal initiation.
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