Evidence map›Paper›PMID 20336452›Full record

ArticleAnalytical and bioanalytical chemistry2010

Capillary array isoelectric focusing with laser-induced fluorescence detection: milli-pH unit resolution and yoctomole mass detection limits in a 32-channel system.

Oluwatosin O Dada, Lauren M Ramsay, Jane A Dickerson, Nathan Cermak, Rong Jiang, Cuiru Zhu, Norman J Dovichi

Abstract readEvaluation Study
In one paragraph

Article in Analytical and bioanalytical chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Review
  6. Article
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  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Oluwatosin O DadaChemistry Department, University of Washington, Seattle, WA 98195-1700, USA.
Lauren M Ramsay
Jane A Dickerson
Nathan Cermak
Rong Jiang
Cuiru Zhu
Norman J Dovichi

Funding

Glycolipid metabolism in single cellsR01NS061767 · NINDS · UNIVERSITY OF WASHINGTON · PI DOVICHI, NORMAN J · 2007 to 2011
$1.8M
NINDS NIH HHS R01 NS061767NINDS NIH HHS R01NS061767
6 · The paper itself

Abstract

We report a multiplexed capillary electrophoresis system employing an array of 32 capillaries with a micromachined sheath-flow cuvette as the detection chamber. The sample streams were simultaneously excited with a 473-nm laser beam, and the fluorescence emission was imaged on a CCD camera with a pair of doublet achromat lens. The instrument produced mass detection limits of 380 +/- 120 yoctomoles for fluorescein in zone electrophoresis. Capillary isoelectric focusing of fluorescent standards produced peaks with an average width of 0.0029 +/- 0.0008 pH. Capillary coating stability limits the reproducibility of the analysis.

Indexed as

Electrophoresis, CapillaryFluoresceinsHydrogen-Ion ConcentrationIsoelectric FocusingLasersLimit of DetectionFluoresceins

Identifiers

PMID20336452
PMCPMC3252051

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.