ArticleProceedings of the National Academy of Sciences of the United States of America2010
Metabolic cycling in single yeast cells from unsynchronized steady-state populations limited on glucose or phosphate.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed.
- Mathematical Modeling for Oscillations Driven by Noncoding RNAs.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Studying the Human Microbiota: Advances in Understanding the Fundamentals, Origin, and Evolution of Biological Timekeeping.International journal of molecular sciences · 2023Review
- Peroxiredoxins couple metabolism and cell division in an ultradian cycle.Nature chemical biology · 2021Article
- Article
- Single-cell imaging and RNA sequencing reveal patterns of gene expression heterogeneity during fission yeast growth and adaptation.Nature microbiology · 2019Article
- Flavin-based metabolic cycles are integral features of growth and division in single yeast cells.Scientific reports · 2018Article
- SCoPE-MS: mass spectrometry of single mammalian cells quantifies proteome heterogeneity during cell differentiation.Genome biology · 2018Article
- A minimal "push-pull" bistability model explains oscillations between quiescent and proliferative cell states.Molecular biology of the cell · 2018Article
- Article
- Article
- Single-cell RNA sequencing reveals intrinsic and extrinsic regulatory heterogeneity in yeast responding to stress.PLoS biology · 2017Article
- Article
- Functional and regulatory profiling of energy metabolism in fission yeast.Genome biology · 2016Article
- ZeitZeiger: supervised learning for high-dimensional data from an oscillatory system.Nucleic acids research · 2016Article
- Nucleosome repositioning underlies dynamic gene expression.Genes & development · 2016Article
- Cell cycle Start is coupled to entry into the yeast metabolic cycle across diverse strains and growth rates.Molecular biology of the cell · 2016Article
- Promoter-Autonomous Functioning in a Controlled Environment using Single Molecule FISH.Scientific reports · 2015Article
- Metabolic Cycles in Yeast Share Features Conserved among Circadian Rhythms.Current biology : CB · 2015Article
- Sequential metabolic phases as a means to optimize cellular output in a constant environment.PloS one · 2015Article
- Genetic basis of haloperidol resistance in Saccharomyces cerevisiae is complex and dose dependent.PLoS genetics · 2014Article
Corrections and comments
- Erratum issued
Authors and funding
11 authors.
Funding
Abstract
Oscillations in patterns of expression of a large fraction of yeast genes are associated with the "metabolic cycle," usually seen only in prestarved, continuous cultures of yeast. We used FISH of mRNA in individual cells to test the hypothesis that these oscillations happen in single cells drawn from unsynchronized cultures growing exponentially in chemostats. Gene-expression data from synchronized cultures were used to predict coincident appearance of mRNAs from pairs of genes in the unsynchronized cells. Quantitative analysis of the FISH results shows that individual unsynchronized cells growing slowly because of glucose limitation or phosphate limitation show the predicted oscillations. We conclude that the yeast metabolic cycle is an intrinsic property of yeast metabolism and does not depend on either synchronization or external limitation of growth by the carbon source.
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