ArticleMolecular biology of the cell2010
MLT-10 defines a family of DUF644 and proline-rich repeat proteins involved in the molting cycle of Caenorhabditis elegans.
Article in Molecular biology of the cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed, 40 citations in OpenAlex.
- High-content phenotypic analysis of a C. elegans recombinant inbred population identifies genetic and molecular regulators of lifespan.Cell reports · 2024Article
- The Caenorhabditis elegans cuticle and precuticle: a model for studying dynamic apical extracellular matrices in vivo.Genetics · 2024Review
- High-content phenotypic analysis ofbioRxiv : the preprint server for biology · 2024Article
- Experimental considerations for study of C. elegans lysosomal proteins.G3 (Bethesda, Md.) · 2023Article
- Condensed tannins act as anthelmintics by increasing the rigidity of the nematode cuticle.Scientific reports · 2022Article
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- Culture and Assay of Large-Scale Mixed-Stage Caenorhabditis elegans Populations.Journal of visualized experiments : JoVE · 2021Article
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- Bacterial diets differentially alter lifespan and healthspan trajectories in C. elegans.Communications biology · 2020Article
- Review
- HSP-4/BiP expression in secretory cells is regulated by a developmental program and not by the unfolded protein response.PLoS biology · 2019Article
- Actin organization and endocytic trafficking are controlled by a network linking NIMA-related kinases to the CDC-42-SID-3/ACK1 pathway.PLoS genetics · 2018Article
- An enhanced C. elegans based platform for toxicity assessment.Scientific reports · 2017Article
- Molting inWorm · 2017Review
- Analysis of a lin-42/period Null Allele Implicates All Three Isoforms in Regulation of Caenorhabditis elegans Molting and Developmental Timing.G3 (Bethesda, Md.) · 2016Article
- Identification of a dTDP-rhamnose biosynthetic pathway that oscillates with the molting cycle in Caenorhabditis elegans.The Biochemical journal · 2016Article
- Caenorhabditis elegans period homolog lin-42 regulates the timing of heterochronic miRNA expression.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Enzymology of the nematode cuticle: A potential drug target?International journal for parasitology. Drugs and drug resistance · 2014Review
- Identification of late larval stage developmental checkpoints in Caenorhabditis elegans regulated by insulin/IGF and steroid hormone signaling pathways.PLoS genetics · 2014Article
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4 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The molting cycle of nematodes involves the periodic synthesis and removal of a collagen-rich exoskeleton, but the underlying molecular mechanisms are not well understood. Here, we describe the mlt-10 gene of Caenorhabditis elegans, which emerged from a genetic screen for molting-defective mutants sensitized by low cholesterol. MLT-10 defines a large family of nematode-specific proteins comprised of DUF644 and tandem P-X(2)-L-(S/T)-P repeats. Conserved nuclear hormone receptors promote expression of the mlt-10 gene in the hypodermis whenever the exoskeleton is remade. Further, a MLT-10::mCherry fusion protein is released from the hypodermis to the surrounding matrices and fluids during molting. The fusion protein is also detected in strands near the surface of animals. Both loss-of-function and gain-of-function mutations of mlt-10 impede the removal of old cuticles. However, the substitution mutation mlt-10(mg364), which disrupts the proline-rich repeats, causes the most severe phenotype. Mutations of mlt-10 are also associated with abnormalities in the exoskeleton and improper development of the epidermis. Thus, mlt-10 encodes a secreted protein involved in three distinct but interconnected aspects of the molting cycle. We propose that the molting cycle of C. elegans involves the dynamic assembly and disassembly of MLT-10 and possibly the paralogs of MLT-10.
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