ArticleMolecular biology of the cell2010
Polarization of the yeast pheromone receptor requires its internalization but not actin-dependent secretion.
Article in Molecular biology of the cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 44 citations in OpenAlex.
- Information bounds on the accuracy of cell polarization.PloS one · 2025Article
- Chemotactic movement of a polarity site enables yeast cells to find their mates.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Exploratory polarization facilitates mating partner selection inMolecular biology of the cell · 2021Article
- Coordinating cell polarization and morphogenesis through mechanical feedback.PLoS computational biology · 2021Article
- GPCR receptor phosphorylation and endocytosis are not necessary to switch polarized growth between internal cues during pheromone response inCommunicative & integrative biology · 2020Article
- Mitotic and pheromone-specific intrinsic polarization cues interfere with gradient sensing inProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Mating yeast cells use an intrinsic polarity site to assemble a pheromone-gradient tracking machine.The Journal of cell biology · 2019Article
- Ratiometric GPCR signaling enables directional sensing in yeast.PLoS biology · 2019Article
- Tracking yeast pheromone receptor Ste2 endocytosis using fluorogen-activating protein tagging.Molecular biology of the cell · 2018Article
- Single-cell profiling screen identifies microtubule-dependent reduction of variability in signaling.Molecular systems biology · 2018Article
- Yeast chemotropism: A paradigm shift in chemical gradient sensing.Cellular logistics · 2017Article
- Unique roles of the unfolded protein response pathway in fungal development and differentiation.Scientific reports · 2016Article
- Gβ promotes pheromone receptor polarization and yeast chemotropism by inhibiting receptor phosphorylation.Science signaling · 2016Article
- A Predictive Model for Yeast Cell Polarization in Pheromone Gradients.PLoS computational biology · 2016Article
- Role of Polarized G Protein Signaling in Tracking Pheromone Gradients.Developmental cell · 2015Article
- Modulation of receptor dynamics by the regulator of G protein signaling Sst2.Molecular biology of the cell · 2015Article
- Cell biology of yeast zygotes, from genesis to budding.Biochimica et biophysica acta · 2015Review
- Sequential logic of polarity determination during the haploid-to-diploid transition in Saccharomyces cerevisiae.Eukaryotic cell · 2014Article
- Specific α-arrestins negatively regulate Saccharomyces cerevisiae pheromone response by down-modulating the G-protein-coupled receptor Ste2.Molecular and cellular biology · 2014Article
- Roles of regulated internalization in the polarization of cell surface receptors.Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference · 2014Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the best understood models of eukaryotic directional sensing, chemotactic cells maintain a uniform distribution of surface receptors even when responding to chemical gradients. The yeast pheromone receptor is also uniformly distributed on the plasma membrane of vegetative cells, but pheromone induces its polarization into "crescents" that cap the future mating projection. Here, we find that in pheromone-treated cells, receptor crescents are visible before detectable polarization of actin cables and that the receptor can polarize in the absence of actin-dependent directed secretion. Receptor internalization, in contrast, seems to be essential for the generation of receptor polarity, and mutations that deregulate this process confer dramatic defects in directional sensing. We also show that pheromone induces the internalization and subsequent polarization of the mating-specific Galpha and Gbeta proteins and that the changes in G protein localization depend on receptor internalization and receptor-Galpha coupling. Our data suggest that the polarization of the receptor and its G protein precedes actin polarization and is important for gradient sensing. We propose that the establishment of receptor/G protein polarity depends on a novel mechanism involving differential internalization and that this serves to amplify the shallow gradient of activated receptor across the cell.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.