ArticleMolecular biology of the cell2010
The spectraplakin Short stop is an actin-microtubule cross-linker that contributes to organization of the microtubule network.
Article in Molecular biology of the cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 56 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
56 citing papers in PubMed, 110 citations in OpenAlex.
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- The Drosophila Nesprin-1 homolog MSP300 is required for muscle autophagy and proteostasis.Journal of cell science · 2024Article
- The Shot CH1 domain recognises a distinct form of F-actin during Drosophila oocyte determination.Development (Cambridge, England) · 2024Article
- A general model for the motion of multivalent cargo interacting with substrates.Journal of the Royal Society, Interface · 2023Article
- Septins mediate a microtubule-actin crosstalk that enables actin growth on microtubules.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- TheMolecular biology of the cell · 2022Article
- Crosstalk between basal extracellular matrix adhesion and building of apical architecture during morphogenesis.Biology open · 2021Review
- Proximity labeling reveals non-centrosomal microtubule-organizing center components required for microtubule growth and localization.Current biology : CB · 2021Article
- Gatekeeper function for Short stop at the ring canals of the Drosophila ovary.Current biology : CB · 2021Article
- A modifier screen identifies regulators of cytoskeletal architecture as mediators of Shroom-dependent changes in tissue morphology.Biology open · 2021Article
- New twists in actin-microtubule interactions.Molecular biology of the cell · 2021Review
- Article
- Microtubules: Evolving roles and critical cellular interactions.Experimental biology and medicine (Maywood, N.J.) · 2019Review
- Microtubules in cell migration.Essays in biochemistry · 2019Review
- Varying crosslinking motifs drive the mesoscale mechanics of actin-microtubule composites.Scientific reports · 2019Article
- Microtubules promote intercellular contractile force transmission during tissue folding.The Journal of cell biology · 2019Article
- The Cytoskeleton-A Complex Interacting Meshwork.Cells · 2019Review
- Review
- Control of microtubule dynamics using an optogenetic microtubule plus end-F-actin cross-linker.The Journal of cell biology · 2018Article
Corrections and comments
- Erratum issuedZadeh, Alireza [corrected to Zadeh, Alireza Dehghani]
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
The dynamics of actin and microtubules are coordinated in a variety of cellular and morphogenetic processes; however, little is known about the molecules mediating this cytoskeletal cross-talk. We are studying Short stop (Shot), the sole Drosophila spectraplakin, as a model actin-microtubule cross-linking protein. Spectraplakins are an ancient family of giant cytoskeletal proteins that are essential for a diverse set of cellular functions; yet, we know little about the dynamics of spectraplakins and how they bridge actin filaments and microtubules. In this study we describe the intracellular dynamics of Shot and a structure-function analysis of its role as a cytoskeletal cross-linker. We find that Shot interacts with microtubules using two different mechanisms. In the cell interior, Shot binds growing plus ends through an interaction with EB1. In the cell periphery, Shot associates with the microtubule lattice via its GAS2 domain, and this pool of Shot is actively engaged as a cross-linker via its NH(2)-terminal actin-binding calponin homology domains. This cross-linking maintains microtubule organization by resisting forces that produce lateral microtubule movements in the cytoplasm. Our results provide the first description of the dynamics of these important proteins and provide key insight about how they function during cytoskeletal cross-talk.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.