ArticleDevelopment (Cambridge, England)2010
Membrane fusion triggers rapid degradation of two gamete-specific, fusion-essential proteins in a membrane block to polygamy in Chlamydomonas.
Article in Development (Cambridge, England), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Molecular determinants of species-specific gamete adhesion triggering HAP2-mediated membrane fusion.Nature communications · 2026Article
- Characteristics of NGenomics, proteomics & bioinformatics · 2023Article
- Eukaryotic fertilization and gamete fusion at a glance.Journal of cell science · 2022Article
- Uncovering an ancestral green ménage à trois: Contributions of Chlamydomonas to the discovery of a broadly conserved triad of plant fertilization proteins.Current opinion in plant biology · 2022Review
- MAR1 links membrane adhesion to membrane merger during cell-cell fusion in Chlamydomonas.Developmental cell · 2021Article
- Species-specific gamete recognition initiates fusion-driving trimer formation by conserved fusogen HAP2.Nature communications · 2021Article
- Molecular Control of Sporophyte-Gametophyte Ontogeny and Transition in Plants.Frontiers in plant science · 2021Review
- Fusexins, HAP2/GCS1 and Evolution of Gamete Fusion.Frontiers in cell and developmental biology · 2021Review
- HAP2-Mediated Gamete Fusion: Lessons From the World of Unicellular Eukaryotes.Frontiers in cell and developmental biology · 2021Review
- New advances and future directions in plant polyspermy.Molecular reproduction and development · 2020Review
- The molecular foundations of zygosis.Cellular and molecular life sciences : CMLS · 2020Review
- Cilia-based peptidergic signaling.PLoS biology · 2019Article
- Article
- Sexual reproduction and sex determination in green algae.Journal of plant research · 2017Review
- Deficiency in Sperm-Egg Protein Interaction as a Major Cause of Fertilization Failure.The Journal of membrane biology · 2017Review
- Article
- Polyspermy barriers: a plant perspective.Current opinion in plant biology · 2017Review
- Mechanisms of endosperm initiation.Plant reproduction · 2016Review
- Dynamic Changes in the Transcriptome and Methylome of Chlamydomonas reinhardtii throughout Its Life Cycle.Plant physiology · 2015Article
- The cytoplasmic domain of the gamete membrane fusion protein HAP2 targets the protein to the fusion site in Chlamydomonas and regulates the fusion reaction.Development (Cambridge, England) · 2015Article
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3 authors.
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Abstract
The plasma membranes of gametes are specialized for fusion, yet, once fusion occurs, in many organisms the new zygote becomes incapable of further membrane fusion reactions. The molecular mechanisms that underlie this loss of fusion capacity (block to polygamy) remain unknown. During fertilization in the green alga Chlamydomonas, the plus gamete-specific membrane protein FUS1 is required for adhesion between the apically localized sites on the plasma membranes of plus and minus gametes that are specialized for fusion, and the minus-specific membrane protein HAP2 is essential for completion of the membrane fusion reaction. HAP2 (GCS1) family members are also required for fertilization in Arabidopsis, and for the membrane fusion reaction in the malaria organism Plasmodium berghei. Here, we tested whether Chlamydomonas gamete fusion triggers alterations in FUS1 and HAP2 and renders the plasma membranes of the cells incapable of subsequent fusion. We find that, even though the fusogenic sites support multi-cell adhesions, triploid zygotes are rare, indicating a fusion-triggered block to the membrane fusion reaction. Consistent with the extinction of fusogenic capacity, both FUS1 and HAP2 are degraded upon fusion. The rapid, fusion-triggered cleavage of HAP2 in zygotes is distinct from degradation occurring during constitutive turnover in gametes. Thus, gamete fusion triggers specific degradation of fusion-essential proteins and renders the zygote incapable of fusion. Our results provide the first molecular explanation for a membrane block to polygamy in any organism.
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