ArticleAnalytical chemistry2010
SlipChip for immunoassays in nanoliter volumes.
Article in Analytical chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 102 citations in OpenAlex.
- Automated, Deep Learning-Enabled Immunoassay Microfluidic Cartridge for Viral Pathogen Detection.Advanced materials technologies · 2025Article
- PDMS SlipChip: Optimizing Sealing, Slipping, and Biocompatibility Using Low-Viscosity Silicone Oils.Micromachines · 2025Article
- A gravity-driven droplet fluidic point-of-care test.Device · 2023Article
- Article
- Advancing microfluidic diagnostic chips into clinical use: a review of current challenges and opportunities.Lab on a chip · 2022Review
- Nanomaterial-assisted microfluidics for multiplex assays.Mikrochimica acta · 2022Review
- Elucidation and control of low and high active populations of alkaline phosphatase molecules for quantitative digital bioassay.Protein science : a publication of the Protein Society · 2021Article
- Composable Microfluidic Plates (cPlate): A Simple and Scalable Fluid Manipulation System for Multiplexed Enzyme-Linked Immunosorbent Assay (ELISA).Analytical chemistry · 2021Article
- Passive micropumping in microfluidics for point-of-care testing.Biomicrofluidics · 2020Review
- Microfluidic SlipChip device for multistep multiplexed biochemistry on a nanoliter scale.Lab on a chip · 2019Article
- Slip-driven microfluidic devices for nucleic acid analysis.Biomicrofluidics · 2019Review
- Microfluidic communicating vessel chip for expedited and automated immunomagnetic assays.Lab on a chip · 2018Article
- Sliding-strip microfluidic device enables ELISA on paper.Biosensors & bioelectronics · 2018Article
- Design and Simulation of Microfluidic Device for Metabolite Screening and Quantitative Monitoring of Drug Uptake in Cancer Cells.Journal of electrical bioimpedance · 2018Article
- Correlative imaging across microscopy platforms using the fast and accurate relocation of microscopic experimental regions (FARMER) method.The Review of scientific instruments · 2017Article
- Detecting explosive molecules from nanoliter solution: A new paradigm of SERS sensing on hydrophilic photonic crystal biosilica.Biosensors & bioelectronics · 2017Article
- Integrative volumetric bar-chart chip for rapid and quantitative point-of-care detection of myocardial infarction biomarkers.Lab on a chip · 2016Article
- Robust dipstick urinalysis using a low-cost, micro-volume slipping manifold and mobile phone platform.Lab on a chip · 2016Article
- Microfluidic platform for separation and extraction of plasma from whole blood using dielectrophoresis.Biomicrofluidics · 2015Article
- Automatic sequential fluid handling with multilayer microfluidic sample isolated pumping.Biomicrofluidics · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
This article describes a SlipChip-based approach to perform bead-based heterogeneous immunoassays with multiple nanoliter-volume samples. As a potential device to analyze the output of the chemistrode, the performance of this platform was tested using low concentrations of biomolecules. Two strategies to perform the immunoassay in the SlipChip were tested: (1) a unidirectional slipping method to combine the well containing a sample with a series of wells preloaded with reagents and (2) a back-and-forth slipping method to introduce a series of reagents to a well containing the sample by reloading and slipping the well containing the reagent. The SlipChips were fabricated with hydrophilic surfaces on the interior of the wells and with hydrophobic surfaces on the face of the SlipChip to enhance filling, transferring, and maintaining aqueous solutions in shallow wells. Nanopatterning was used to increase the hydrophobic nature of the SlipChip surface. Magnetic beads containing the capture antibody were efficiently transferred between wells and washed by serial dilution. An insulin immunoenzymatic assay showed a detection of limit of approximately 13 pM. A total of 48 droplets of nanoliter volume were analyzed in parallel, including an on-chip calibration. The design of the SlipChip is flexible to accommodate other types of immunoassays, both heterogeneous and homogeneous. This work establishes the possibility of using SlipChip-based immunoassays in small volumes for a range of possible applications, including analysis of plugs from a chemistrode, detection of molecules from single cells, and diagnostic monitoring.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.