ArticleWorld journal of gastroenterology2010
Expression and functional characterization of platelet-derived growth factor receptor-like gene.
Article in World journal of gastroenterology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 26 citations in OpenAlex.
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- Exploring the oncogenic potential of Aiolos in lung cancer through OTUB1-mediated ubiquitination.Heliyon · 2024Article
- Genetic analysis of platelet-derived growth factor receptor-like gene (Archives animal breeding · 2024Article
- A temporal single cell transcriptome atlas of zebrafish anterior segment development.Scientific reports · 2023Article
- Comprehensive Analysis of the Prognostic Significance of Hsa-miR-100-5p and Its Related Gene Signature in Stomach Adenocarcinoma.Frontiers in cell and developmental biology · 2021Article
- Trimester-Specific Associations of Prenatal Lead Exposure With Infant Cord Blood DNA Methylation at Birth.Epigenetics insights · 2020Article
- Novel candidates in early-onset familial colorectal cancer.Familial cancer · 2020Article
- Genetic variation in SLC7A2 interacts with calcium and magnesium intakes in modulating the risk of colorectal polyps.The Journal of nutritional biochemistry · 2017Article
- The innate immune response in fetal lung mesenchymal cells targets VEGFR2 expression and activity.American journal of physiology. Lung cellular and molecular physiology · 2017Article
- RNA-seq transcriptome analysis of formalin fixed, paraffin-embedded canine meningioma.PloS one · 2017Article
- Positional plasticity in regenerating Amybstoma mexicanum limbs is associated with cell proliferation and pathways of cellular differentiation.BMC developmental biology · 2015Article
- Article
- Solute carrier protein family may involve in radiation-induced radioresistance of non-small cell lung cancer.Journal of cancer research and clinical oncology · 2011Article
- Interactions between glucocorticoid treatment and cis-regulatory polymorphisms contribute to cellular response phenotypes.PLoS genetics · 2011Article
- Effects of α-Particle Radiation on DNA Methylation in Human Hepatocytes.Dose-response : a publication of International Hormesis SocietyArticle
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo investigate the role of platelet-derived growth factor receptor-like gene (PDGFRL) in the anti-cancer therapy for colorectal cancers (CRC).
methodsPDGFRL mRNA and protein levels were measured by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry in CRC and colorectal normal tissues. PDGFRL prokaryotic expression vector was carried out in Escherichia coli (E. coli), and purified by immobilized metal affinity chromatography. The effect of PDGFRL protein on CRC HCT-116 cells was detected by 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT), clone counting, cell cycle, and wound healing assay.
resultsBoth RT-PCR and immunohistochemistry showed that the expression of PDGFRL in colorectal normal tissues was higher than in cancer tissues. Recombinant pET22b-PDGFRL prokaryotic expression vector was successfully expressed in E. coli, and the target protein was expressed in the form of inclusion bodies. After purification and refolding, recombinant human PDGFRL (rhPDGFRL) could efficiently inhibit the proliferation and invasion of CRC HCT-116 cells detected by MTT, clone counting and wound healing assay. Moreover, rhPDGFRL arrested HCT-116 cell cycling at the G0/G1 phase.
conclusionPDGFRL is a potential gene for application in the anti-cancer therapy for CRC.
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