ArticleCellular and molecular bioengineering2009
Validation, In-Depth Analysis, and Modification of the Micropipette Aspiration Technique.
Article in Cellular and molecular bioengineering, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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Who cites it
6 citing papers in PubMed.
- Design and Analysis of a Two-Degree-of-Freedom Inertial Piezoelectric Platform.Materials (Basel, Switzerland) · 2025Article
- Biomechanics of Neutrophil Tethers.Life (Basel, Switzerland) · 2021Review
- Characterization of cytoplasmic viscosity of hundreds of single tumour cells based on micropipette aspiration.Royal Society open science · 2019Article
- Endothelial Surface Protrusion by a Point Force.Biophysical journal · 2016Article
- The constitutive equation for membrane tether extraction.Annals of biomedical engineering · 2010Article
- Unfolding the A2 domain of von Willebrand factor with the optical trap.Biophysical journal · 2010Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The micropipette aspiration technique (MAT) has been successfully applied to many studies in cell adhesion such as leukocyte-endothelium interactions. However, this technique has never been validated experimentally and it has been only employed to impose constant forces. In this study, we validated the force measurement of the MAT with the optical trap and analyzed two technical issues of the MAT, force-transducer offset and cell-micropipette gap, with finite element simulation. We also modified the MAT so that increasing or decreasing forces can be applied. With the modified MAT, we studied tether extraction from endothelial cells by pulling single tethers at increasing velocities and constant force loading rates. Before the onset of tether extraction, an apparently-linear surface protrusion of a few hundred nanometers was observed, which is likely related to membrane receptors pulling on the underlying cytoskeleton. The strength of the modified MAT lies in its capability and consistency to apply a wide range of force loading rates from several piconewtons per second up to thousands of piconewtons per second. With this modification, the MAT becomes more versatile in the study of single molecule and single cell biophysics.
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Registered trials
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