ArticleThe Journal of biological chemistry2010
Multiple functions as lipase, steryl ester hydrolase, phospholipase, and acyltransferase of Tgl4p from the yeast Saccharomyces cerevisiae.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 92 citations in OpenAlex.
- Transcriptomic Analysis of Lipid Accumulation in Mucor circinelloides WJ11 Under Submerged and Solid-State Fermentation.Applied biochemistry and biotechnology · 2025Article
- Engineering yeast for tailored fatty acid profiles.Applied microbiology and biotechnology · 2025Review
- Triacylglycerol mobilization underpins mitochondrial stress recovery.Nature cell biology · 2025Article
- Toxoplasma gondii serine hydrolases regulate parasite lipid mobilization during growth and replication within the host.Cell chemical biology · 2021Article
- Phosphatidic acid biosynthesis in the model organism yeast Saccharomyces cerevisiae - a survey.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2021Review
- Protein Phosphatase Sit4 Affects Lipid Droplet Synthesis and Soraphen A Resistance Independent of Its Role in Regulating Elongator Dependent tRNA Modification.Biomolecules · 2018Article
- Lipid droplet-mediated lipid and protein homeostasis in budding yeast.FEBS letters · 2018Review
- Patatin-like phospholipases in microbial infections with emerging roles in fatty acid metabolism and immune regulation by Apicomplexa.Molecular microbiology · 2018Review
- Ymr210wp leads to the accumulation of phospholipids and steryl esters in yeast.Bioinformation · 2017Article
- Lipase genes in Mucor circinelloides: identification, sub-cellular location, phylogenetic analysis and expression profiling during growth and lipid accumulation.Journal of industrial microbiology & biotechnology · 2016Article
- Regulation of the yeast triacylglycerol lipases Tgl4p and Tgl5p by the presence/absence of nonpolar lipids.Molecular biology of the cell · 2016Article
- Monoacylglycerol Lipases Act as Evolutionarily Conserved Regulators of Non-oxidative Ethanol Metabolism.The Journal of biological chemistry · 2016Article
- Computational Modeling of Lipid Metabolism in Yeast.Frontiers in molecular biosciences · 2016Article
- Morphogenesis checkpoint kinase Swe1 is the executor of lipolysis-dependent cell-cycle progression.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- A defect of the vacuolar putative lipase Atg15 accelerates degradation of lipid droplets through lipolysis.Autophagy · 2015Article
- Article
- Lipid Acyl Chain Remodeling in Yeast.Lipid insights · 2015Review
- Modifications of the C terminus affect functionality and stability of yeast triacylglycerol lipase Tgl3p.The Journal of biological chemistry · 2014Article
- Adipose triglyceride lipase activity is inhibited by long-chain acyl-coenzyme A.Biochimica et biophysica acta · 2014Article
- Review: biogenesis of the multifunctional lipid droplet: lipids, proteins, and sites.The Journal of cell biology · 2014Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Triacylglycerol (TAG) hydrolysis, membrane lipid biosynthesis, and lipid turnover are largely interlinked processes. In yeast, TAG is mobilized by three TAG lipases named Tgl3p, Tgl4p, and Tgl5p, which are localized to lipid particles/droplets. These TAG lipases posses a conserved GXSXG motif that is characteristic of hydrolytic enzymes. Here, we demonstrated that the yeast TAG lipase Tgl4p, the functional ortholog of the adipose TAG lipase, ATGL, catalyzes multiple functions in lipid metabolism. An extended domain and motif search analysis revealed that Tgl4p bears not only a lipase consensus domain but also a conserved motif for calcium-independent phospholipase A(2). We show that Tgl4p exhibits TAG lipase, steryl ester hydrolase, and phospholipase A(2) activities, but surprisingly it also catalyzed the acyl-CoA-dependent acylation of lysophosphatidic acid to phosphatidic acid (PA). Heterologous overexpression of Tgl4p in Pichia pastoris increased total phospholipid and specifically PA synthesis. Moreover, deletion of TGL4 in Saccharomyces cerevisiae showed an altered pattern of phosphatidylcholine and PA molecular species. Altogether, our data suggest that yeast Tgl4p functions as a hydrolytic enzyme in lipid degradation but also contributes to fatty acid channeling and phospholipid remodeling.
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