ArticleDevelopment (Cambridge, England)2010
Direct activation of Shroom3 transcription by Pitx proteins drives epithelial morphogenesis in the developing gut.
Article in Development (Cambridge, England), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 65 citations in OpenAlex.
- Large-scale, spatially resolved panoramic CRISPR screening in native tissue environments using Perturb-DBiT.Nature biotechnology · 2026Article
- Mapping Shroom3 expression across the adult mouse.Gene expression patterns : GEP · 2025Article
- Shroom3 facilitates optic fissure closure via tissue alignment and reestablishment of apical-basal polarity during epithelial fusion.Developmental biology · 2025Review
- CommonGenome research · 2025Article
- Apical constriction requires patterned apical surface remodeling to synchronize cellular deformation.eLife · 2025Article
- Systematic mediation and interaction analyses of kidney function genetic loci in a general population study.PloS one · 2025Article
- Article
- The Critical Role of the Shroom Family Proteins in Morphogenesis, Organogenesis and Disease.Phenomics (Cham, Switzerland) · 2024Review
- CellNeighborEX: deciphering neighbor-dependent gene expression from spatial transcriptomics data.Molecular systems biology · 2023Article
- The Good and the Bad of SHROOM3 in Kidney Development and Disease: A Narrative Review.Canadian journal of kidney health and disease · 2023Review
- Optogenetic control of apical constriction induces synthetic morphogenesis in mammalian tissues.Nature communications · 2022Article
- Discovery of new therapeutic targets in ovarian cancer through identifying significantly non-mutated genes.Journal of translational medicine · 2022Article
- Somitic mesoderm morphogenesis is necessary for neural tube closure during Xenopus development.Frontiers in cell and developmental biology · 2022Article
- Programmed and self-organized flow of information during morphogenesis.Nature reviews. Molecular cell biology · 2021Review
- SHROOM3 is downstream of the planar cell polarity pathway and loss-of-function results in congenital heart defects.Developmental biology · 2020Article
- Convergent extension in the amphibian, Xenopus laevis.Current topics in developmental biology · 2020Review
- Folic acid modifies the shape of epithelial cells during morphogenesis via a Folr1 and MLCK dependent mechanism.Biology open · 2019Article
- The RhoGEF protein Plekhg5 regulates apical constriction of bottle cells during gastrulation.Development (Cambridge, England) · 2018Article
- Transcriptional regulation of cell shape during organ morphogenesis.The Journal of cell biology · 2018Review
- The left-right asymmetry of liver lobation is generated by Pitx2c-mediated asymmetries in the hepatic diverticulum.Developmental biology · 2018Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Individual cell shape changes are essential for epithelial morphogenesis. A transcriptional network for epithelial cell shape change is emerging in Drosophila, but this area remains largely unexplored in vertebrates. The distinction is important as so far, key downstream effectors of cell shape change in Drosophila appear not to be conserved. Rather, Shroom3 has emerged as a central effector of epithelial morphogenesis in vertebrates, driving both actin- and microtubule-based cell shape changes. To date, the morphogenetic role of Shroom3 has been explored only in the neural epithelium, so the broad expression of this gene raises two important questions: what are the requirements for Shroom3 in non-neural tissues and what factors control Shroom3 transcription? Here, we show in Xenopus that Shroom3 is essential for cell shape changes and morphogenesis in the developing vertebrate gut and that Shroom3 transcription in the gut requires the Pitx1 transcription factor. Moreover, we show that Pitx proteins directly activate Shroom3 transcription, and we identify Pitx-responsive regulatory elements in the genomic DNA upstream of Shroom3. Finally, we show that ectopic expression of Pitx proteins is sufficient to induce Shroom3-dependent cytoskeletal reorganization and epithelial cell shape change. These data demonstrate new breadth to the requirements for Shroom3 in morphogenesis, and they also provide a cell-biological basis for the role of Pitx transcription factors in morphogenesis. More generally, these results provide a foundation for deciphering the transcriptional network that underlies epithelial cell shape change in developing vertebrates.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.