ArticleBMC evolutionary biology2010
Quasispecies-like behavior observed in catalytic RNA populations evolving in a test tube.
Article in BMC evolutionary biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Genome Sequencing of Historical Encephalomyocarditis Viruses from South Africa Links the Historical 1993/4 Savanna Elephant (Pathogens (Basel, Switzerland) · 2024Article
- Ribozyme Mutagenic Evolution: Mechanisms of Survival.Origins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life · 2021Article
- Chemical Fueling Enables Molecular Complexification of Self-Replicators*.Angewandte Chemie (International ed. in English) · 2021Article
- In vitro selections with RNAs of variable length converge on a robust catalytic core.Nucleic acids research · 2021Article
- Social Networking of Quasi-Species Consortia drive Virolution via Persistence.AIMS microbiology · 2021Review
- Towards open-ended evolution in self-replicating molecular systems.Beilstein journal of organic chemistry · 2017Review
- From formamide to RNA, the path is tenuous but continuous.Life (Basel, Switzerland) · 2015Article
- Quasispecies in population of compositional assemblies.BMC evolutionary biology · 2014Article
- Limits of neutral drift: lessons from the in vitro evolution of two ribozymes.Journal of molecular evolution · 2014Article
- Rethinking quasispecies theory: From fittest type to cooperative consortia.World journal of biological chemistry · 2013Review
- Article
- Viral quasispecies evolution.Microbiology and molecular biology reviews : MMBR · 2012Review
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2 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundDuring the RNA World, molecular populations were probably very small and highly susceptible to the force of strong random drift. In conjunction with Muller's Ratchet, this would have imposed difficulties for the preservation of the genetic information and the survival of the populations. Mechanisms that allowed these nascent populations to overcome this problem must have been advantageous.
resultsUsing continuous in vitro evolution experimentation with an increased mutation rate imposed by MnCl2, it was found that clonal 100-molecule populations of ribozymes clearly exhibit certain characteristics of a quasispecies. This is the first time this has been seen with a catalytic RNA. Extensive genotypic sampling from two replicate lineages was gathered and phylogenetic networks were constructed to elucidate the structure of the evolving RNA populations. A common distribution was found in which a mutant sequence was present at high frequency, surrounded by a cloud of mutant with lower frequencies. This is a typical distribution of quasispecies. Most of the mutants in these clouds were connected by short Hamming distance values, indicating their close relatedness.
conclusionsThe quasispecies nature of mutant RNA clouds facilitates the recovery of genotypes under pressure of being removed from the population by random drift. The empirical populations therefore evolved a genotypic resiliency despite a high mutation rate by adopting the characteristics of quasispecies, implying that primordial RNA pools could have used this strategy to avoid extinction.
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