ArticleMolecular oral microbiology2010
DNA from Porphyromonas gingivalis and Tannerella forsythia induce cytokine production in human monocytic cell lines.
Article in Molecular oral microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 54 citations in OpenAlex.
- Assessment of the role of Trichomonas tenax in the etiopathogenesis of human periodontitis: A systematic review.PloS one · 2019Pooled it
- Porphyromonas gingivalis Outer Membrane Vesicles-Associated DNA Triggers Inflammation by Inducing IL-6 in Astrocytes.Molecular oral microbiology · 2026Article
- Role of immunostimulatory deoxycytidylate-phosphate-deoxyguanylate (CpG) motifs in oral bacteria associated with oral diseases.Journal of oral microbiology · 2025Article
- Analysis of periodontal status in Polish patients with rheumatoid arthritis treated with biological therapies.Reumatologia · 2024Article
- Nanoparticulate cell-free DNA scavenger for treating inflammatory bone loss in periodontitis.Nature communications · 2022Article
- Oral microbial extracellular DNA initiates periodontitis through gingival degradation by fibroblast-derived cathepsin K in mice.Communications biology · 2022Article
- CpG Single-Site Methylation Regulates TLR2 Expression in Proinflammatory PBMCs From Apical Periodontitis Individuals.Frontiers in immunology · 2022Article
- Periodontitis and Gestational Diabetes Mellitus: A Potential Inflammatory Vicious Cycle.International journal of molecular sciences · 2021Review
- Role of oral pathogens in the pathogenesis of intracranial aneurysm: review of existing evidence and potential mechanisms.Neurosurgical review · 2021Review
- Systemic and Extraradicular Bacterial Translocation in Apical Periodontitis.Frontiers in cellular and infection microbiology · 2021Article
- Porphyromonas gingivalis in the tongue biofilm is associated with clinical outcome in rheumatoid arthritis patients.Clinical and experimental immunology · 2018Article
- Soluble epoxide hydrolase inhibitor promotes immunomodulation to inhibit bone resorption.Journal of periodontal research · 2018Article
- Interplay of Toll-Like Receptor 9, Myeloid Cells, and Deubiquitinase A20 in Periodontal Inflammation.Infection and immunity · 2017Article
- Infections as a cause of autoimmune rheumatic diseases.Auto- immunity highlights · 2016Review
- Microbial Nucleic Acid Sensing in Oral and Systemic Diseases.Journal of dental research · 2016Review
- The novel association between red complex of oral microbe and body mass index in healthy Japanese: a population based cross-sectional study.Journal of clinical biochemistry and nutrition · 2015Article
- Toll-Like Receptor 9-Mediated Inflammation Triggers Alveolar Bone Loss in Experimental Murine Periodontitis.Infection and immunity · 2015Article
- Chemokine function in periodontal disease and oral cavity cancer.Frontiers in immunology · 2015Review
- Relationship of clinical and microbiological variables in patients with type 1 diabetes mellitus and periodontitis.Medical science monitor : international medical journal of experimental and clinical research · 2014Article
- Novel inflammatory pathways in periodontitis.Advances in dental research · 2014Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Toll-like receptor 9 (TLR9) expression is increased in periodontally diseased tissues compared with healthy sites indicating a possible role of TLR9 and its ligand, bacterial DNA (bDNA), in periodontal disease pathology. Here, we determine the immunostimulatory effects of periodontal bDNA in human monocytic cells (THP-1). THP-1 cells were stimulated with DNA of two putative periodontal pathogens: Porphyromonas gingivalis and Tannerella forsythia. The role of TLR9 in periodontal bDNA-initiated cytokine production was determined either by blocking TLR9 signaling in THP-1 cells with chloroquine or by measuring IL-8 production and nuclear factor-kappaB (NF-kappaB) activation in HEK293 cells stably transfected with human TLR9. Cytokine production (IL-1beta, IL-6, and TNF-alpha) was increased significantly in bDNA-stimulated cells compared with controls. Chloroquine treatment of THP-1 cells decreased cytokine production, suggesting that TLR9-mediated signaling pathways are operant in the recognition of DNA from periodontal pathogens. Compared with native HEK293 cells, TLR9-transfected cells demonstrated significantly increased IL-8 production (P < 0.001) and NF-kappaB activation in response to bDNA, further confirming the role of TLR9 in periodontal bDNA recognition. The results of PCR arrays demonstrated upregulation of proinflammatory cytokine and NF-kappaB genes in response to periodontal bDNA in THP-1 cells, suggesting that cytokine induction is through NF-kappaB activation. Hence, immune responses triggered by periodontal bacterial nucleic acids may contribute to periodontal disease pathology by inducing proinflammatory cytokine production through the TLR9 signaling pathway.
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Registered trials
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