ArticleMolecular oral microbiology2010
A metaproteomic analysis of the human salivary microbiota by three-dimensional peptide fractionation and tandem mass spectrometry.
Article in Molecular oral microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Mass Spectrometry-Based Oral Meta-Omics Analysis-What We Know and What We Don't.Advances in experimental medicine and biology · 2026Review
- Proteomic snapshot of saliva samples predicts new pathways implicated in SARS-CoV-2 pathogenesis.Clinical proteomics · 2024Article
- Genome-Resolved Metaproteomics Decodes the Microbial and Viral Contributions to Coupled Carbon and Nitrogen Cycling in River Sediments.mSystems · 2022Article
- Vascular Calcification and the Gut and Blood Microbiome in Chronic Kidney Disease Patients on Peritoneal Dialysis: A Pilot Study.Biomolecules · 2022Article
- Impact of different oral treatments on the composition of the supragingival plaque microbiome.Journal of oral microbiology · 2022Article
- Current State and Challenges of the Global Outcomes of Dental Caries Research in the Meta-Omics Era.Frontiers in cellular and infection microbiology · 2022Review
- In-Depth Metaproteomics Analysis of Oral Microbiome for Lung Cancer.Research (Washington, D.C.) · 2022Article
- Bioinspired caries preventive strategy via customizable pellicles of saliva-derived protein/peptide constructs.Scientific reports · 2021Article
- High-throughput sequencing identifies salivary microbiota in Chinese caries-free preschool children with primary dentition.Journal of Zhejiang University. Science. B · 2021Article
- [Research progress on carbohydrate active enzymes of human microbiome].Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology · 2019Review
- Metaproteomics analysis of microbial diversity of human saliva and tongue dorsum in young healthy individuals.Journal of oral microbiology · 2019Article
- Disseminating Metaproteomic Informatics Capabilities and Knowledge Using the Galaxy-P Framework.Proteomes · 2018Article
- Comparative analysis of Salivette® and paraffin gum preparations for establishment of a metaproteomics analysis pipeline for stimulated human saliva.Journal of oral microbiology · 2018Article
- Ugly ducklings-the dark side of plastic materials in contact with potable water.NPJ biofilms and microbiomes · 2018Article
- Salivary pellets induce a pro-inflammatory response involving the TLR4-NF-kB pathway in gingival fibroblasts.BMC oral health · 2016Article
- What are We Learning and What Can We Learn from the Human Oral Microbiome Project?Current oral health reports · 2016Article
- Monitoring Perinatal Gut Microbiota in Mouse Models by Mass Spectrometry Approaches: Parental Genetic Background and Breastfeeding Effects.Frontiers in microbiology · 2016Article
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- Beyond microbial community composition: functional activities of the oral microbiome in health and disease.Microbes and infection · 2015Article
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Authors and funding
5 authors.
Funding
Abstract
Metagenomics uses gene expression patterns to understand the taxonomy and metabolic activities of microbial communities. Metaproteomics applies the same approach to community proteomes. Previously, we used a novel three-dimensional peptide separation method to identify over 2000 salivary proteins. This study used those data to carry out the first metaproteomic analysis of the human salivary microbiota. The metagenomic software MEGAN generated a phylogenetic tree, which was checked against the Human Oral Microbiome Database (HOMD). Pathway analyses were performed with the Clusters of Orthologous Groups and MetaCyc databases. Thirty-seven per cent of the peptides were identifiable only at the level of cellular organisms or bacteria. The rest were distributed among five bacterial phyla (61%), archea (0.5%), and viruses (0.8%); 29% were assignable at the genus level, and most belonged to Streptococcus (17%). Eleven per cent of all peptides could be assigned to species. Most taxa were represented in HOMD and they included well-known species such as periodontal pathogens. However, there also were 'exotic' species including aphid endosymbionts; plant, water, and soil bacteria; extremophiles; and archea. The pathway analysis indicated that peptides were linked to translation (37%), followed by glycolysis (19%), amino acid metabolism (8%), and energy production (8%). The taxonomic structure of the salivary metaproteome is very diverse but is dominated by streptococci. 'Exotic' species may actually represent close relatives that have not yet been sequenced. Salivary microbes appear to be actively engaged in protein synthesis, and the pathway analysis is consistent with the metabolism of salivary glycoproteins.
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