ArticleJournal of the American Chemical Society2010
Enzyme-amplified array sensing of proteins in solution and in biofluids.
Article in Journal of the American Chemical Society, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
54 citing papers in PubMed, 212 citations in OpenAlex.
- Colorimetric Biosensors: Advancements in Nanomaterials and Cutting-Edge Detection Strategies.Biosensors · 2025Review
- Tuning multispectral fluorescence quantum dot-based identification of short-length amyloid β peptides by applying Cu(II) ions.Mikrochimica acta · 2024Article
- Development of Optical Differential Sensing Based on Nanomaterials for Biological Analysis.Biosensors · 2024Review
- Controlled Bio-Orthogonal Catalysis Using Nanozyme-Protein Complexes via Modulation of Electrostatic Interactions.Materials (Basel, Switzerland) · 2024Article
- Nanoparticles with intermediate hydrophobicity polarize macrophages to plaque-specific Mox phenotype via Nrf2 and HO-1 activation.Journal of hazardous materials · 2024Article
- In situ Tracking of Exoenzyme Activity Using Droplet Luminescence Concentrators for Ratiometric Detection of Bacteria.ACS sensors · 2023Article
- Article
- Carbocyanine-Based Optical Sensor Array for the Discrimination of Proteins and Rennet Samples Using Hypochlorite Oxidation.Sensors (Basel, Switzerland) · 2023Article
- Role of Ionic Strength in the Formation of Stable Supramolecular Nanoparticle-Protein Conjugates for Biosensing.International journal of molecular sciences · 2022Article
- Molecular Probes, Chemosensors, and Nanosensors for Optical Detection of Biorelevant Molecules and Ions in Aqueous Media and Biofluids.Chemical reviews · 2022Review
- Portable electrochemical micro-workstation platform for simultaneous detection of multiple Alzheimer's disease biomarkers.Mikrochimica acta · 2022Article
- Lipophilicity of Cationic Ligands Promotes Irreversible Adsorption of Nanoparticles to Lipid Bilayers.ACS nano · 2021Article
- Evolution of Supramolecular Systems Towards Next-Generation Biosensors.Frontiers in chemistry · 2021Review
- Article
- Application of Chemometrics in Biosensing: A Review.Biosensors · 2020Review
- Fluorescent Materials With Aggregation-Induced Emission Characteristics for Array-Based Sensing Assay.Frontiers in chemistry · 2020Review
- Array-Based Discriminative Optical Biosensors for Identifying Multiple Proteins in Aqueous Solution and Biofluids.Frontiers in chemistry · 2020Review
- Stereospecific modulation of dimeric rhodopsin.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Water-Dispersible and Biocompatible Iron Carbide Nanoparticles with High Specific Absorption Rate.ACS nano · 2019Article
- Protein⁻Polyelectrolyte Interaction: Thermodynamic Analysis Based on the Titration MethodPolymers · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
We have developed an enzyme-nanoparticle sensor array where the sensitivity is amplified through enzymatic catalysis. In this approach cationic gold nanoparticles are electrostatically bound to an enzyme (beta-galactosidase, beta-Gal), inhibiting enzyme activity. Analyte proteins release the beta-Gal, restoring activity and providing an amplified readout of the binding event. Using this strategy we have been able to identify proteins in buffer at a concentration of 1 nM, substantially lower than current strategies for array-based protein sensing. Moreover, we have obtained identical sensitivity in studies where the proteins are spiked into the complex protein matrix provided by desalted human urine ( approximately 1.5 muM total protein; spiked protein concentrations were 0.067% of the overall protein concentration), demonstrating the potential of the method for diagnostic applications.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.