ArticleBiomedical microdevices2010
A microfluidic imaging chamber for the direct observation of chemotactic transmigration.
Article in Biomedical microdevices, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed.
- Review
- Of vascular defense, hemostasis, cancer, and platelet biology: an evolutionary perspective.Cancer metastasis reviews · 2022Review
- Versatile and High-throughput Force Measurement Platform for Dorsal Cell Mechanics.Scientific reports · 2019Article
- Fluorescence enhanced lab-on-a-chip patterned using a hybrid technique of femtosecond laser direct writing and anodized aluminum oxide porous nanostructuring.Nanoscale advances · 2019Article
- Cancer Cells Invade Confined Microchannels via a Self-Directed Mesenchymal-to-Amoeboid Transition.Nano letters · 2019Article
- Neuregulin-1 is a chemoattractant and chemokinetic molecule for trunk neural crest cells.Developmental dynamics : an official publication of the American Association of Anatomists · 2018Article
- Platelet Metabolism and Other Targeted Drugs; Potential Impact on Immunotherapy.Frontiers in oncology · 2018Review
- Platelet "first responders" in wound response, cancer, and metastasis.Cancer metastasis reviews · 2017Review
- Cancer cell motility: lessons from migration in confined spaces.Nature reviews. Cancer · 2017Review
- A microfluidic device for isolation and characterization of transendothelial migrating cancer cells.Biomicrofluidics · 2017Article
- Increased cancer stem cell invasion is mediated by myosin IIB and nuclear translocation.Oncotarget · 2016Article
- Engineered Models of Confined Cell Migration.Annual review of biomedical engineering · 2016Review
- On-Chip Quantitative Measurement of Mechanical Stresses During Cell Migration with Emulsion Droplets.Scientific reports · 2016Article
- Cell Blebbing in Confined Microfluidic Environments.PloS one · 2016Article
- Non-muscle myosin IIB is critical for nuclear translocation during 3D invasion.The Journal of cell biology · 2015Article
- An open access microfluidic device for the study of the physical limits of cancer cell deformation during migration in confined environments.Microelectronic engineering · 2015Article
- Microfluidic gradient device for studying mesothelial cell migration and the effect of chronic carbon nanotube exposure.Journal of micromechanics and microengineering : structures, devices, and systems · 2015Article
- Small-molecule axon-polarization studies enabled by a shear-free microfluidic gradient generator.Lab on a chip · 2014Article
- A microfluidic-based genetic screen to identify microbial virulence factors that inhibit dendritic cell migration.Integrative biology : quantitative biosciences from nano to macro · 2014Article
- Microdevice integrating innate and adaptive immune responses associated with antigen presentation by dendritic cells.RSC advances · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
To study the roles of nonmuscle myosin II (NM-II) during invasive cell migration, microfluidic migration chambers have been designed and fabricated using photo- and soft-lithography microfabrication techniques. The chamber consists of two channels separated by a vertical barrier with multiple bays of pores with widths varying from 6 microm to 16 microm, and lengths varying from 25 microm to 50 microm. The cells are plated in the channel on one side of the barrier while a chemoattractant is flowed through the channel on the other side of the barrier. In these chambers, cells can be observed with transmitted light or fluorescence optics while they chemotax through various sized pores that impose differential mechanical resistance to transmigration. As an initial test of this device, we compared breast-cancer cell chemotactic transmigration through different pore sizes with and without inhibition of NM-II. Two distinct rates were observed as cells attempted to pull their nucleus through the smaller pores, and the faster nuclear transit mode was critically dependent on NM-II motor activity. The ability to monitor cells as they chemotax through pores of different dimensions within a single experimental system provides novel information on how pore size affects cell morphology and migration rate, providing a dramatic improvement of imaging potential relative to other in vitro transmigration systems such as Boyden chambers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.