ArticleHistochemistry and cell biology2010
The characterization of the first anti-mouse Muc6 antibody shows an increased expression of the mucin in pancreatic tissue of Cftr-knockout mice.
Article in Histochemistry and cell biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 23 citations in OpenAlex.
- Identification of early genes in the pathophysiology of fibrotic interstitial lung disease in a new model of pulmonary fibrosis.Cellular and molecular life sciences : CMLS · 2025Article
- A porous cervical mucus plug leads to preterm birth induced by experimental vaginal infection in mice.iScience · 2022Article
- Actions of glucagon-like peptide-1 receptor ligands in the gut.British journal of pharmacology · 2022Review
- Fenretinide Reduces Intestinal Mucin-2-Positive Goblet Cells in Chronic Alcohol Abuse.Pharmacology · 2022Article
- Muc5b-deficient mice develop early histological lung abnormalities.Biology open · 2019Article
- Muc5b is mainly expressed and sialylated in the nasal olfactory epithelium whereas Muc5ac is exclusively expressed and fucosylated in the nasal respiratory epithelium.Histochemistry and cell biology · 2019Article
- Ductal Mucus Obstruction and Reduced Fluid Secretion Are Early Defects in Chronic Pancreatitis.Frontiers in physiology · 2018Article
- In vivo imaging of the Muc5b gel-forming mucin.Scientific reports · 2017Article
- Preclinical mouse model to monitor live Muc5b-producing conjunctival goblet cell density under pharmacological treatments.PloS one · 2017Article
- Pancreatic pathophysiology in cystic fibrosis.The Journal of pathology · 2016Review
- Delivery of a mucin domain enriched in cysteine residues strengthens the intestinal mucous barrier.Scientific reports · 2015Article
- Animal models of gastrointestinal and liver diseases. Animal models of cystic fibrosis: gastrointestinal, pancreatic, and hepatobiliary disease and pathophysiology.American journal of physiology. Gastrointestinal and liver physiology · 2015Review
- Studies of mucus in mouse stomach, small intestine, and colon. II. Gastrointestinal mucus proteome reveals Muc2 and Muc5ac accompanied by a set of core proteins.American journal of physiology. Gastrointestinal and liver physiology · 2013Article
- CFTR, mucins, and mucus obstruction in cystic fibrosis.Cold Spring Harbor perspectives in medicine · 2012Review
- CFTR-mediated Cl(-) transport in the acinar and duct cells of rabbit lacrimal gland.Current eye research · 2012Article
- Article
- Abnormal expression of Muc5b in Cftr-null mice and in mammary tumors of MMTV-ras mice.Histochemistry and cell biology · 2011Article
- The extraordinarily complex but highly structured organization of intestinal mucus-gel unveiled in multicolor images.PloS one · 2011Article
- Histochemistry and cell biology: the annual review 2010.Histochemistry and cell biology · 2011Review
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gel-forming mucins are large high-molecular weight secreted O-glycoproteins responsible for the gel-properties of the mucus blanket. Five orthologous gel-forming mucins have been cloned in human and mouse. Among them, the mucin MUC6 has been less studied, particularly in rodents and no anti rodent-Muc6 antibody has been reported yet. In order to further study Muc6 in mice, our aims were to obtain a specific Muc6 antibody, to validate it and to test it in Cftr deficient mice. A polyclonal serum named CP4 was isolated from a rabbit immunized by a mouse Muc6 peptide. In Western blot experiments, the antibody detected a high-molecular weight molecule secreted by the gastric tissue. Using immunohistochemistry, we showed that the antibody reacted strongly with deep glands of duodenum and ileum and mucous neck cells of gastric body. CP4 also recognized Muc6 protein secreted at the surface of the stomach and renal collecting tubules. The centroacinar cells of pancreatic tissue also reacted with the antibody. Cftr-/- mice showed a higher expression of Muc6 at both protein and RNA levels compared with their control Cftr+/+ littermates suggesting that as in the human disease, Muc6 may contribute to the formation of materials that block pancreatic acini and ducts in mouse models of cystic fibrosis. The rabbit anti-mouse Muc6 polyclonal antibody seems highly specific to the mouse mucin and will be useful to study pancreatic pathology in cystic fibrosis.
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