ArticleNucleic acids research1991
Construction of an ordered clone bank and systematic analysis of the whole transcripts of chromosome VI of Saccharomyces cerevisiae.
Article in Nucleic acids research, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Novel PI(4)P 5-kinase homologue, Fab1p, essential for normal vacuole function and morphology in yeast.Molecular biology of the cell · 1995Article
- Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: analysis of the genes in the FUN38-MAK16-SPO7 region.Journal of bacteriology · 1994Article
- Location and characterization of autonomously replicating sequences from chromosome VI of Saccharomyces cerevisiae.Molecular and cellular biology · 1993Article
- Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6 kilobase pairs.Genetics · 1993Article
- Correlation between observed transcripts and sequenced ORFs of chromosome III of Saccharomyces cerevisiae.Nucleic acids research · 1993Article
- An ordered clone bank for chromosome I of Saccharomyces cerevisiae.Journal of bacteriology · 1992Article
- Physical dissection and characterization of chromosomes V and VIII of Saccharomyces cerevisiae.Nucleic acids research · 1992Article
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2 authors.
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Abstract
By comparing sequences of restriction enzyme cleavage sites and their distance data, we sorted 384 lambda phage clones containing segments of chromosome VI of S. cerevisiae and constructed an ordered clone bank for this chromosome. The physical length of this bank is 269.7 kb. The bank contains the entire chromosome including the left telomere, but it is not certain whether it contains the right telomere as well. To estimate the number of genes present on this chromosome, we performed a series of Northern hybridization experiments using 157 restriction enzyme fragments prepared from the bank as hybridization probes and total poly(A)+ RNA from vegetatively growing cells. Thus, 97 distinct transcripts were identified. The relative abundance levels of individual transcripts were measured by comparing their band intensity with that of the RPO41 transcript. It was found that the transcripts from the genes located in the telomeric and centromeric regions are less abundant as compared to those from the genes in the central regions of both arms.
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