ArticleProceedings of the National Academy of Sciences of the United States of America2010
Combinatorial libraries against libraries for selecting neoepitope activation-specific antibodies.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
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- Integration of phage and yeast display platforms: A reliable and cost effective approach for binning of peptides as displayed on-phage.PloS one · 2020Article
- Functional detection of botulinum neurotoxin serotypes A to F by monoclonal neoepitope-specific antibodies and suspension array technology.Scientific reports · 2019Article
- Geometry and expression enhance enrichment of functional yeast-displayed ligands via cell panning.Biotechnology and bioengineering · 2016Article
- Recent advances in the field of anti-cancer immunotherapy.BBA clinical · 2015Review
- Recombinant renewable polyclonal antibodies.mAbs · 2015Article
- Discovering aptamers by cell-SELEX against human soluble growth factors ectopically expressed on yeast cell surface.PloS one · 2014Article
- Isolation of αL I domain mutants mediating firm cell adhesion using a novel flow-based sorting method.Protein engineering, design & selection : PEDS · 2013Article
- Cells and cell lysates: a direct approach for engineering antibodies against membrane proteins using yeast surface display.Methods (San Diego, Calif.) · 2013Review
- Cell surface assembly of HIV gp41 six-helix bundles for facile, quantitative measurements of hetero-oligomeric interactions.Journal of the American Chemical Society · 2012Article
- Diversity in genetic in vivo methods for protein-protein interaction studies: from the yeast two-hybrid system to the mammalian split-luciferase system.Microbiology and molecular biology reviews : MMBR · 2012Review
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- Cross-domain inhibition of TACE ectodomain.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Review
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
A systematic approach to the discovery of conformation-specific antibodies or those that recognize activation-induced neoepitopes in signaling molecules and enzymes will be a powerful tool in developing antibodies for basic science and therapy. Here, we report the isolation of antibody antagonists that preferentially bind activated integrin Mac-1 (alpha(M)beta(2)) and are potent in blocking neutrophil adhesion and migration. A novel strategy was developed for this task, consisting of yeast surface display of Mac-1 inserted (I) domain library, directed evolution to isolate active mutants of the I domain, and screening of phage display of human antibody library against the active I domain in yeast. Enriched antibody library was then introduced into yeast surface two-hybrid system for final quantitative selection of antibodies from monomeric antigen-antibody interaction. This led to highly efficient isolation of intermediate to high affinity antibodies, which preferentially reacted with the active I domain, antagonized the I domain binding to intercellular adhesion molecule (ICAM)-1, complement C3 fragment iC3b, and fibronectin, and potently inhibited neutrophil migration on fibrinogen. The strategy demonstrated herein can be broadly applicable to developing antibodies against modular domains that switch between inactive and active conformations, particularly toward the discovery of antibody antagonists in therapeutic and diagnostic applications.
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