ArticleProceedings of the National Academy of Sciences of the United States of America2010
Transcriptome and metabolome profiling of field-grown transgenic barley lack induced differences but show cultivar-specific variances.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed.
- The Effect of Arbuscular Mycorrhizal Fungus and Phosphorus Treatment on Root Metabolome ofPlants (Basel, Switzerland) · 2025Article
- Genetically modified soybean lines exhibit less transcriptomic variation compared to natural varieties.GM crops & food · 2023Article
- Editorial: Arbuscular mycorrhiza-mediated augmentation of plant secondary metabolite production.Frontiers in plant science · 2023Article
- Comparison of the Phenotypic Performance, Molecular Diversity, and Proteomics in Transgenic Rice.Plants (Basel, Switzerland) · 2022Article
- Investigation of Possible Changes Induced by RNA Silencing in Some Leaf Metabolites of Transgenic Sugar Beet Events.Food chemistry. Molecular sciences · 2022Article
- Review
- Entailing the Next-Generation Sequencing and Metabolome for Sustainable Agriculture by Improving Plant Tolerance.International journal of molecular sciences · 2022Review
- Transcriptomic Analysis of Mature Transgenic Poplar Expressing the Transcription FactorFrontiers in bioengineering and biotechnology · 2022Article
- Article
- Primary metabolic processes as drivers of leaf ageing.Cellular and molecular life sciences : CMLS · 2021Review
- Metabolic Analysis RevealsMetabolites · 2021Article
- Evaluation on reprogramed biological processes in transgenic maize varieties using transcriptomics and metabolomics.Scientific reports · 2021Article
- Plant breeding involving genetic engineering does not result in unacceptable unintended effects in rice relative to conventional cross-breeding.The Plant journal : for cell and molecular biology · 2020Article
- Sequencing of two transgenic early-flowering poplar lines confirmed vector-free single-locus T-DNA integration.Transgenic research · 2020Article
- Genetic variation assessment of stacked-trait transgenic maize via conventional breeding.BMC plant biology · 2019Article
- Unintended effects of transgenic rice revealed by transcriptome and metabolism.GM crops & food · 2019Article
- Recent advances in the characterization of plant transcriptomes in response to drought, salinity, heat, and cold stress.F1000Research · 2019Review
- Trichoderma for climate resilient agriculture.World journal of microbiology & biotechnology · 2017Review
- Reverse Genetics and High Throughput Sequencing Methodologies for Plant Functional Genomics.Current genomics · 2016Article
- Development of a construct-based risk assessment framework for genetic engineered crops.Transgenic research · 2016Review
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of the present study was to assess possible adverse effects of transgene expression in leaves of field-grown barley relative to the influence of genetic background and the effect of plant interaction with arbuscular mycorrhizal fungi. We conducted transcript profiling, metabolome profiling, and metabolic fingerprinting of wild-type accessions and barley transgenics with seed-specific expression of (1,3-1, 4)-beta-glucanase (GluB) in Baronesse (B) as well as of transgenics in Golden Promise (GP) background with ubiquitous expression of codon-optimized Trichoderma harzianum endochitinase (ChGP). We found more than 1,600 differential transcripts between varieties GP and B, with defense genes being strongly overrepresented in B, indicating a divergent response to subclinical pathogen challenge in the field. In contrast, no statistically significant differences between ChGP and GP could be detected based on transcriptome or metabolome analysis, although 22 genes and 4 metabolites were differentially abundant when comparing GluB and B, leading to the distinction of these two genotypes in principle component analysis. The coregulation of most of these genes in GluB and GP, as well as simple sequence repeat-marker analysis, suggests that the distinctive alleles in GluB are inherited from GP. Thus, the effect of the two investigated transgenes on the global transcript profile is substantially lower than the effect of a minor number of alleles that differ as a consequence of crop breeding. Exposing roots to the spores of the mycorrhizal Glomus sp. had little effect on the leaf transcriptome, but central leaf metabolism was consistently altered in all genotypes.
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