ArticleInfection and immunity2010
Genome sequencing of recent clinical Chlamydia trachomatis strains identifies loci associated with tissue tropism and regions of apparent recombination.
Article in Infection and immunity, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
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Who cites it
82 citing papers in PubMed, 119 citations in OpenAlex.
- Chromosome Editing Using the Lambda Red Recombinase System in Chlamydia trachomatis and Chlamydia muridarum.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Visual Screening of Genetic Polymorphisms inSensors (Basel, Switzerland) · 2026Article
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- Genomic insights into local-scale evolution of ocularMicrobial genomics · 2024Article
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- Genetic Characterization of Non-International journal of molecular sciences · 2023Article
- Patterns of within-host spread ofbioRxiv : the preprint server for biology · 2023Article
- Recent advances in genetic systems in obligate intracellular human-pathogenic bacteria.Frontiers in cellular and infection microbiology · 2023Review
- Patterns of within-host spread ofFrontiers in microbiology · 2023Article
- Genomic Analysis of MSM Rectal Chlamydia trachomatis Isolates Identifies Predicted Tissue-Tropic Lineages Generated by Intraspecies Lateral Gene Transfer-Mediated Evolution.Infection and immunity · 2022Article
- The Impact of Lateral Gene Transfer inFrontiers in cellular and infection microbiology · 2022Review
- Completing the Genome Sequence ofPathogens (Basel, Switzerland) · 2021Article
- Article
- A broad-spectrum cloning vector that exists as both an integrated element and a free plasmid in Chlamydia trachomatis.PloS one · 2021Article
- Article
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- Chromosomal Recombination Targets inJournal of bacteriology · 2019Article
- Whole-genome sequencing of ocular Chlamydia trachomatis isolates from Gadarif State, Sudan.Parasites & vectors · 2019Article
22 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The human pathogen Chlamydia trachomatis exists as multiple serovariants that have distinct organotropisms for different tissue sites. Culture and epidemiologic data have demonstrated that serovar G is more prevalent, while serovar E is less prevalent, for rectal isolates from men having sex with men (MSM). The relative prevalence of these serovars is the opposite for isolates from female cervical infections. In contrast, the prevalence of serovar J isolates is approximately the same at the different tissue sites, and these isolates are the only C-class strains that are routinely cultured from MSM populations. These correlations led us to hypothesize that polymorphisms in open reading frame (ORF) sequences correlate with the different tissue tropisms of these serovars. To explore this possibility, we sequenced and compared the genomes of clinical anorectal and cervical isolates belonging to serovars E, G, and J and compared these genomes with each other, as well as with a set of previously sequenced genomes. We then used PCR- and restriction digestion-based genotyping assays performed with a large collection of recent clinical isolates to show that polymorphisms in ORFs CT144, CT154, and CT326 were highly associated with rectal tropism in serovar G isolates and that polymorphisms in CT869 and CT870 were associated with tissue tropism across all serovars tested. The genome sequences collected were also used to identify regions of likely recombination in recent clinical strains. This work demonstrated that whole-genome sequencing along with comparative genomics is an effective approach for discovering variable loci in Chlamydia spp. that are associated with clinical presentation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.