ArticlePhilosophical transactions of the Royal Society of London. Series B, Biological sciences2010
Rate and effects of spontaneous mutations that affect fitness in mutator Escherichia coli.
Article in Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- The adaptive state determines the impact of mutations on evolving populations.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Evolutionary Advantage of Cell Size Control.Physical review letters · 2025Article
- Article
- Reversions mask the contribution of adaptive evolution in microbiomes.bioRxiv : the preprint server for biology · 2024Article
- Comparative genomics hints at dispensability of multiple essential genes in two Escherichia coli L-form strains.Bioscience reports · 2023Article
- Are mutations usually deleterious? A perspective on the fitness effects of mutation accumulation.Evolutionary ecology · 2022Article
- A Single Nucleotide Change in theApplied and environmental microbiology · 2022Article
- Precise measurement of the fitness effects of spontaneous mutations by droplet digital PCR in Burkholderia cenocepacia.Genetics · 2021Article
- Evolution of Pseudomonas aeruginosa toward higher fitness under standard laboratory conditions.The ISME journal · 2021Article
- Article
- Mutation bias and GC content shape antimutator invasions.Nature communications · 2019Article
- The Mutator Phenotype: Adapting Microbial Evolution to Cancer Biology.Frontiers in genetics · 2019Review
- Experimental Design, Population Dynamics, and Diversity in Microbial Experimental Evolution.Microbiology and molecular biology reviews : MMBR · 2018Review
- High mutation rates limit evolutionary adaptation in Escherichia coli.PLoS genetics · 2018Article
- Article
- Extremely Rare Polymorphisms in Saccharomyces cerevisiae Allow Inference of the Mutational Spectrum.PLoS genetics · 2017Article
- The Nonstationary Dynamics of Fitness Distributions: Asexual Model with Epistasis and Standing Variation.Genetics · 2016Article
- The Fitness Effects of Spontaneous Mutations Nearly Unseen by Selection in a Bacterium with Multiple Chromosomes.Genetics · 2016Article
- Fitness is strongly influenced by rare mutations of large effect in a microbial mutation accumulation experiment.Genetics · 2014Article
- Rates of fitness decline and rebound suggest pervasive epistasis.Evolution; international journal of organic evolution · 2014Article
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3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Knowledge of the mutational parameters that affect the evolution of organisms is of key importance in understanding the evolution of several characteristics of many natural populations, including recombination and mutation rates. In this study, we estimated the rate and mean effect of spontaneous mutations that affect fitness in a mutator strain of Escherichia coli and review some of the estimation methods associated with mutation accumulation (MA) experiments. We performed an MA experiment where we followed the evolution of 50 independent mutator lines that were subjected to repeated bottlenecks of a single individual for approximately 1150 generations. From the decline in mean fitness and the increase in variance between lines, we estimated a minimum mutation rate to deleterious mutations of 0.005 (+/-0.001 with 95% confidence) and a maximum mean fitness effect per deleterious mutation of 0.03 (+/-0.01 with 95% confidence). We also show that any beneficial mutations that occur during the MA experiment have a small effect on the estimate of the rate and effect of deleterious mutations, unless their rate is extremely large. Extrapolating our results to the wild-type mutation rate, we find that our estimate of the mutational effects is slightly larger and the inferred deleterious mutation rate slightly lower than previous estimates obtained for non-mutator E. coli.
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