ArticleThe Journal of biological chemistry2010
LVIS553 transcriptional regulator specifically recognizes novobiocin as an effector molecule.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 20 citations in OpenAlex.
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- Identification of Biomarkers for Systemic Distribution of Nanovesicles FromFrontiers in immunology · 2021Article
- Purification and partial characterization of LdtP, a cell envelope modifying enzyme in Liberibacter asiaticus.BMC microbiology · 2018Article
- Zinc is an inhibitor of the LdtR transcriptional activator.PloS one · 2018Article
- Functional characterization of LotP from Liberibacter asiaticus.Microbial biotechnology · 2017Article
- Identification of the Tolfenamic Acid Binding Pocket in PrbP fromFrontiers in microbiology · 2017Article
- Functional Analysis of the Citrate Activator CitO from Enterococcus faecalis Implicates a Divalent Metal in Ligand Binding.Frontiers in microbiology · 2016Article
- Drug Repurposing: Tolfenamic Acid Inactivates PrbP, a Transcriptional Accessory Protein inFrontiers in microbiology · 2016Article
- Identification of a Ligand Binding Pocket in LdtR from Liberibacter asiaticus.Frontiers in microbiology · 2015Article
- H2O2 production rate in Lactobacillus johnsonii is modulated via the interplay of a heterodimeric flavin oxidoreductase with a soluble 28 Kd PAS domain containing protein.Frontiers in microbiology · 2015Article
- A dual role of the transcriptional regulator TstR provides insights into cyanide detoxification in Lactobacillus brevis.Molecular microbiology · 2014Article
- The transcriptional activator LdtR from 'Candidatus Liberibacter asiaticus' mediates osmotic stress tolerance.PLoS pathogens · 2014Article
- Identification of a small molecule that modifies MglA/SspA interaction and impairs intramacrophage survival of Francisella tularensis.PloS one · 2013Article
- Anaerobic p-coumarate degradation by Rhodopseudomonas palustris and identification of CouR, a MarR repressor protein that binds p-coumaroyl coenzyme A.Journal of bacteriology · 2012Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
In this study we aimed to identify small molecules with high affinity involved in the allosteric regulation of LVIS553, a MarR member from Lactobacillus brevis ATCC367. Using high throughput screening, novobiocin was found to specifically bind LVIS553 with a K(D) = 33.8 +/- 2.9 microM consistent with a biologically relevant ligand. Structure guided site-directed mutagenesis identified Lys(9) as a key residue in novobiocin recognition. The results found in vitro were correlated in vivo. An increased tolerance to the antibiotic was observed when LVIS553 and the downstream putative transport protein LVIS552 were either expressed in a low copy plasmid in L. brevis or as a single copy chromosomal insertion in Bacillus subtilis. We provide evidence that LVIS553 is involved in the specific regulation of a new mechanism of tolerance to novobiocin.
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