ArticleJournal of diabetes science and technology2010
Maximizing deoxyribonucleic acid yield from dried blood spots.
Article in Journal of diabetes science and technology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Concordance of next generation sequence-based and sequence specific oligonucleotide probe-based HLA-DRB1 genotyping.Human immunology · 2015Article
- Visual automated fluorescence electrophoresis provides simultaneous quality, quantity, and molecular weight spectra for genomic DNA from archived neonatal blood spots.The Journal of molecular diagnostics : JMD · 2013Article
- Comparative analytical utility of DNA derived from alternative human specimens for molecular autopsy and diagnostics.The Journal of molecular diagnostics : JMD · 2012Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
backgroundOne source of deoxyribonucleic acid (DNA) for genetic studies is the utilization of dried blood spots stored on paper cards (Guthrie cards) collected shortly after birth. These cards represent an important source of material for epidemiologic and population-based genetic studies. Extraction of DNA from these cards can lead to variable amounts of recovered DNA. We report here results of our efforts to maximize yield from this valuable, but nonrenewable, resource.
methodCommercial methods of DNA extraction from blood cards were used, and protocol modifications were introduced that enhanced DNA yield.
resultsUse of a commercial solvent prior to DNA extraction steps gave greater yields than extraction without the solvent. Modification of the elution step by use of prewarmed extraction buffer and a soaking step at an elevated temperature increased yield by 6- to 10-fold.
conclusionsThe modified DNA extraction method yielded as much as 660 ng of DNA from a single 5-mm-diameter punch of a blood spot card. The DNA performed well in downstream, polymerase chain reaction-based applications.
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