ArticleProtein science : a publication of the Protein Society2010
Dimer-monomer equilibrium of human thymidylate synthase monitored by fluorescence resonance energy transfer.
Article in Protein science : a publication of the Protein Society, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Disordered N-Terminal Tail "Wags the Dog" in Human Thymidylate Synthase.Biochemistry · 2026Article
- Article
- Interfacial Peptides as Affinity Modulating Agents of Protein-Protein Interactions.Biomolecules · 2022Review
- Designing Chimeric Molecules for Drug Discovery by Leveraging Chemical Biology.Journal of medicinal chemistry · 2020Review
- PDB_Amyloid: an extended live amyloid structure list from the PDB.FEBS open bio · 2019Article
- New Insight into the Octamer of TYMS Stabilized by Intermolecular Cys43-Disulfide.International journal of molecular sciences · 2018Article
- Crystal structure of the active form of native human thymidylate synthase in the absence of bound substrates.Acta crystallographica. Section F, Structural biology communications · 2017Article
- Intracellular quantitative detection of human thymidylate synthase engagement with an unconventional inhibitor using tetracysteine-diarsenical-probe technology.Scientific reports · 2016Article
- Hotspots in an obligate homodimeric anticancer target. Structural and functional effects of interfacial mutations in human thymidylate synthase.Journal of medicinal chemistry · 2015Article
- Alanine mutants of the interface residues of human thymidylate synthase decode key features of the binding mode of allosteric anticancer peptides.Journal of medicinal chemistry · 2015Article
- Analysis of mRNA recognition by human thymidylate synthase.Bioscience reports · 2014Article
- EmrE dimerization depends on membrane environment.Biochimica et biophysica acta · 2014Article
- Neglected role of cAMP receptor protein monomer.Molecular biology reports · 2012Article
- Protein-protein interface-binding peptides inhibit the cancer therapy target human thymidylate synthase.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Caught in self-interaction: evolutionary and functional mechanisms of protein homooligomerization.Physical biology · 2011Review
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6 authors.
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Abstract
An ad hoc bioconjugation/fluorescence resonance energy transfer (FRET) assay has been designed to spectroscopically monitor the quaternary state of human thymidylate synthase dimeric protein. The approach enables the chemoselective engineering of allosteric residues while preserving the native protein functions through reversible masking of residues within the catalytic site, and is therefore suitable for activity/oligomerization dual assay screenings. It is applied to tag the two subunits of human thymidylate synthase at cysteines 43 and 43' with an excitation energy donor/acceptor pair. The dimer-monomer equilibrium of the enzyme is then characterized through steady-state fluorescence determination of the intersubunit resonance energy transfer efficiency.
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